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鸡颅骨骨细胞缝隙连接细胞间通讯的荧光漂白恢复分析。

The Analysis of Gap Junctional Intercellular Communication Among Osteocytes in Chick Calvariae by Fluorescence Recovery After Photobleaching.

机构信息

Department of Orthodontics, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan.

Research Fellow of Japan Society for the Promotion of Science, Tokyo, Japan.

出版信息

Methods Mol Biol. 2021;2346:215-223. doi: 10.1007/7651_2020_322.

Abstract

This chapter describes the use of fluorescence recovery after photobleaching (FRAP) for analyzing gap junctional intercellular communication (GJIC) among osteocytes in chick calvariae by confocal laser scanning microscope.

摘要

本章描述了利用荧光漂白恢复后(FRAP)技术,通过共聚焦激光扫描显微镜分析鸡颅骨骨细胞间缝隙连接细胞间通讯(GJIC)的应用。

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