Chen Qing, Zeng Qun-li, Lu De-qiang, Jiang Huai
Zhejiang Provincial Key Laboratory of Bioelectromagnetics, Zhejiang University School of Medicine, Hangzhou 310031, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2004 Jan;38(1):8-10.
To explore the effect of millimeter wave (MW) at low power density on gap junctional intercellular communication (GJIC) in human keratinocytes (HaCaTs).
Fluorescence recovery after photobleaching (FRAP) technique was employed to determine effect of 30.16 GHz MW exposure at 1.0 and 3.5 mW/cm(2) on GJIC with laser confocal scanning microscope.
FRAP analysis revealed that 12-O-tetradecanoylphorbol-13-acetate (TPA) at a dose of 5 microg/L could inhibit GJIC in HaCaTs. Fluorescence recovery rate fell from (55 +/- 17)% in the controls to (34 +/- 13)% after photobleaching, with a very significant difference (P < 0.001). Exposure to MW alone for one hour at either 1.0 mW/cm(2) or 3.5 mW/cm(2) did not affect GJIC, with fluorescence recovery rates of (52 +/- 16)% and (50 +/- 17)%, respectively. GJIC suppression induced by TPA was weakened by MW combined with 5 microg/L TPA treatment for one hour, which could be partially recovered by exposure to 1.0 mW/cm(2) MW with fluorescence recovery rate of (47 +/- 16)%, P < 0.01, and fully recovered by exposure to 3.5 mW/cm(2) MW with fluorescence recovery rate of (50 +/- 16)%, P < 0.001, with a very significant difference.
GJIC suppression induced by TPA could be eliminated or diminished by exposure to millimeter wave in HaCaTs.
探讨低功率密度毫米波（MW）对人角质形成细胞（HaCaTs）缝隙连接细胞间通讯（GJIC）的影响。
采用光漂白后荧光恢复（FRAP）技术，通过激光共聚焦扫描显微镜测定30.16 GHz MW在1.0和3.5 mW/cm²暴露剂量下对GJIC的影响。
FRAP分析显示，5 μg/L剂量的12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）可抑制HaCaTs中的GJIC。光漂白后荧光恢复率从对照组的（55±17）%降至（34±13）%，差异非常显著（P < 0.001）。在1.0 mW/cm²或3.5 mW/cm²单独暴露MW 1小时均不影响GJIC，荧光恢复率分别为（52±16）%和（50±17）%。MW与5 μg/L TPA联合处理1小时可减弱TPA诱导的GJIC抑制，暴露于1.0 mW/cm² MW可部分恢复，荧光恢复率为（47±16）%，P < 0.01，暴露于3.5 mW/cm² MW可完全恢复，荧光恢复率为（50±16）%，P < 0.001，差异非常显著。
在HaCaTs中，暴露于毫米波可消除或减轻TPA诱导的GJIC抑制。
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