单细胞 RNA 测序在胰腺癌和内分泌胰腺中的应用。
Application of Single-Cell RNA Sequencing in Pancreatic Cancer and the Endocrine Pancreas.
机构信息
Department of Hepato-Biliary-Pancreatic Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, China.
出版信息
Adv Exp Med Biol. 2020;1255:143-152. doi: 10.1007/978-981-15-4494-1_12.
The pancreas is a complex organ composed of an endocrine (pancreatic islets) and an exocrine portion. This mixed cell population has resulted in an implacable barrier to exploring the detailed mechanism and function of each cell type in previous investigative approaches. In recent years, single-cell RNA sequencing (scRNA-seq) technologies have provided in-depth analysis of cell heterogeneity in the pancreas and in pancreatic cancer. It is especially effective in cell-type-specific molecule identification and detection of interactions between cancer cells and the stromal microenvironment. To date, numerous reports have described the application of scRNA-seq in studies of pancreatic islets and pancreatic cancer. The aim of this paper is to review recent advances of pancreatic transcriptomics and pancreatic cancer using scRNA-seq strategies.
胰腺是一个由内分泌（胰岛）和外分泌部分组成的复杂器官。这种混合细胞群体导致在以前的研究方法中，探索每种细胞类型的详细机制和功能存在着难以逾越的障碍。近年来，单细胞 RNA 测序（scRNA-seq）技术为胰腺和胰腺癌中的细胞异质性提供了深入分析。它在鉴定细胞类型特异性分子和检测癌细胞与基质微环境之间的相互作用方面特别有效。迄今为止，已有大量报道描述了 scRNA-seq 在胰岛和胰腺癌研究中的应用。本文旨在综述使用 scRNA-seq 策略的胰腺转录组学和胰腺癌的最新进展。
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