Institute of Chinese Sturgeon, China Three Gorges Corporation, Yichang, Hubei, 443100, China; Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Institute of Chinese Sturgeon, China Three Gorges Corporation, Yichang, Hubei, 443100, China.
Dev Comp Immunol. 2021 Jan;114:103872. doi: 10.1016/j.dci.2020.103872. Epub 2020 Sep 17.
Citrobacter freundii is one of the important bacterial diseases responsible for disease outbreaks to wild and cultured fishes globally. However, no known empirical research has focused on exploring relationships between immune response after C. freundii infection in sturgeons. In this study, C. freundii was isolated and identified from artificially breeding Chinese sturgeon, and global measurement of transcriptome response to C. freundii infection in head-kidney and spleen of A. sinensis were conducted to the acknowledgement of the potential mechanisms of pathogen-host interaction triggered by the bacterial infection. In total, differentially expressed genes which significantly associated with immune responses were found to be participated in antigen processing and presentation (MHC I, MHC II, HspA1, Hsp90A, Hsp70, CTSL, and CTSE), and acute phase response (serotransferrin and CP), as well as changing of other immune-related cytokine, such as chemokine and interferon, which proving their reacting and regulatory role during the response of thehost against C. freundii infection in fish. C. freundii can cause serious disease in sturgeon species was first reported in this study, and innate immune responses to C. freundii infection in this study will be conducive to understand the defense mechanisms and making appropriate prevention strategies in A. sinensis aquaculture operations.
弗氏柠檬酸杆菌是引起全球野生和养殖鱼类疾病爆发的重要细菌病原之一。然而，目前还没有已知的经验性研究集中于探索鲟鱼感染弗氏柠檬酸杆菌后的免疫反应之间的关系。在本研究中，从人工养殖的中华鲟中分离并鉴定出弗氏柠檬酸杆菌，并对中华鲟头肾和脾脏中对弗氏柠檬酸杆菌感染的转录组反应进行了全面测量，以了解细菌感染引发的病原体-宿主相互作用的潜在机制。总的来说，发现与免疫反应显著相关的差异表达基因参与了抗原加工和呈递（MHC I、MHC II、HspA1、Hsp90A、Hsp70、CTSL 和 CTSE）以及急性期反应（转铁蛋白和 CP），以及其他免疫相关细胞因子（如趋化因子和干扰素）的变化，证明它们在宿主对鱼类弗氏柠檬酸杆菌感染的反应中具有反应和调节作用。本研究首次报道弗氏柠檬酸杆菌可引起鲟鱼严重疾病，本研究中对弗氏柠檬酸杆菌感染的先天免疫反应将有助于了解中华鲟水产养殖中的防御机制并制定适当的预防策略。
