Biochemistry Department, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology (MUST), 6th October City, Giza, Egypt.
Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Anal Biochem. 2020 Nov 15;609:113967. doi: 10.1016/j.ab.2020.113967. Epub 2020 Sep 18.
Diabetic nephropathy (DN) is a major leading cause of kidney failure. So, early detection of DN by assessing urinary microRNAs (miRNAs) expression may be of clinical value. In this study, the diagnostic value of two urinary miRNAs (miR-210 & miR-34a) as biomarkers for diagnosis of DN was assessed using a simple colorimetric gold nanoparticle (AuNP) assay and real-time PCR. MiR-(210 & 34a) were markedly up-regulated in DN groups (micro-albuminuric and macro-albuminuric groups) compared to the non-albuminuric group and healthy controls. The sensitivity and specificity for the qualitative detection of urinary miR-(210 & 34a) using the AuNP assay were (78% and 72%) & (81% and 69%), respectively, which were consistent with the results of real-time PCR. There was a highly significant correlation between urinary miR-(210 & 34a) detected by either qRT-PCR or qualitative AuNP assay. Accordingly, this simple AuNP assay may be considered a valid test for the detection of these two urinary miRNAs as potential biomarkers that can aid in the noninvasive diagnosis of DN.
糖尿病肾病（DN）是导致肾衰竭的主要原因之一。因此，通过评估尿 microRNAs（miRNAs）表达来早期检测 DN 可能具有临床价值。在这项研究中，使用简单的比色金纳米颗粒（AuNP）测定法和实时 PCR 评估了两种尿 miRNA（miR-210 和 miR-34a）作为诊断 DN 的生物标志物的诊断价值。与非白蛋白尿组和健康对照组相比，DN 组（微量白蛋白尿和大量白蛋白尿组）中 miR-（210 和 34a）明显上调。使用 AuNP 测定法定性检测尿 miR-（210 和 34a）的灵敏度和特异性分别为（78% 和 72%）和（81% 和 69%），与实时 PCR 的结果一致。通过 qRT-PCR 或定性 AuNP 测定法检测到的尿 miR-（210 和 34a）之间存在高度显著的相关性。因此，这种简单的 AuNP 测定法可被视为检测这两种潜在生物标志物的尿液 miRNA 的有效测试，有助于非侵入性诊断 DN。
