Research Center for Guangxi Reproductive Medicine, First Affiliated Hospital of Guangxi Medical University, Nanning, China; Reproductive Medicine Center, the Affiliated Hospital, Guilin Medical College, Guilin, China; Reproductive Medicine Center, Taihe Hospital, Hubei Medical University, Shiyan, China.
Reproductive Medicine Center, the Affiliated Hospital, Guilin Medical College, Guilin, China.
Life Sci. 2020 Nov 1;260:118439. doi: 10.1016/j.lfs.2020.118439. Epub 2020 Sep 17.
This study aims to investigate the effects of intrauterine perfusion of granulocyte colony-stimulating factor (G-CSF) on a thin-endometrium rat model.
Twenty rats in two groups of 10 were used. Group I was perfused with normal saline (NS) in the right uterine horn and 95% ethanol in the left one. Group II was bilaterally perfused with 95% ethanol into the uterine horns. After three estrous cycles, Group II was perfused with NS in the right uterine horn and G-CSF (30 μg/kg) in the left one. Hematoxylin-eosin (HE) and immunohistochemistry (IHC) staining were used to detect changes in endometrial thickness and expression of cytokeratin 19 (CK19) and vimentin (Vim). The relative expression levels of vascular endothelial growth factor (Vegf) and leukemia inhibitory factor (Lif) were also tested via reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) and Western-blot analyses.
G-CSF treatment significantly increased the thickness of the endometrium in the 95% ethanol-induced thin-endometrium rat model. The expression levels of endometrial glandular epithelial cell marker for CK19 and stromal cell marker Vim were augmented in the G-CSF-treated group compared with the control group. Moreover, G-CSF treatment stimulated the expression of VEGF and LIF in the 95% ethanol-induced thin-endometrium rat model.
G-CSF intrauterine perfusion improved endometrial receptivity in the thin-endometrium rat model by stimulating endometrial proliferation and angiogenesis.
本研究旨在探讨宫腔内灌注粒细胞集落刺激因子（G-CSF）对薄型子宫内膜大鼠模型的影响。
将 20 只大鼠分为两组，每组 10 只。第 1 组右侧子宫角用生理盐水（NS）灌注，左侧用 95%乙醇灌注。第 2 组双侧子宫角用 95%乙醇灌注。经过三个动情周期后，第 2 组右侧子宫角用 NS 灌注，左侧用 G-CSF（30μg/kg）灌注。采用苏木精-伊红（HE）和免疫组织化学（IHC）染色检测子宫内膜厚度和细胞角蛋白 19（CK19）和波形蛋白（Vim）表达的变化。通过逆转录定量实时聚合酶链反应（RT-qPCR）和 Western-blot 分析检测血管内皮生长因子（Vegf）和白血病抑制因子（Lif）的相对表达水平。
G-CSF 治疗显著增加了 95%乙醇诱导的薄型子宫内膜大鼠模型的子宫内膜厚度。与对照组相比，G-CSF 治疗组子宫内膜腺上皮细胞标志物 CK19 和间质细胞标志物 Vim 的表达水平增加。此外，G-CSF 治疗刺激了 95%乙醇诱导的薄型子宫内膜大鼠模型中 VEGF 和 LIF 的表达。
G-CSF 宫腔内灌注通过刺激子宫内膜增殖和血管生成，改善了薄型子宫内膜大鼠模型的子宫内膜容受性。
