[针对单个组蛋白组分的抗血清的制备及特性]
[Preparation and characteristics of antisera to individual histone fractions].
作者信息
Zalenskaia I A, Zelenskaia E O, Mikhaĭlov V M
出版信息
Tsitologiia. 1977 Feb;19(2):238-42.
PMID:329506
Abstract
A method is described of preparation and characterization of antisera to pure individual histone fractions not conjugated with other proteins or haptens. Rabbits were given two injections of the antigen and the whole immunization schedule took only three weeks. The antisera were characterized by immunofluorescent technique using mouse liver sections and smears of rat liver nuclei.
摘要
本文描述了一种制备和鉴定针对未与其他蛋白质或半抗原结合的纯个体组蛋白组分的抗血清的方法。给兔子注射两次抗原，整个免疫程序仅需三周。使用小鼠肝脏切片和大鼠肝细胞核涂片通过免疫荧光技术对这些抗血清进行了鉴定。
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