Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China.
Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China.
Fish Shellfish Immunol. 2020 Nov;106:1052-1066. doi: 10.1016/j.fsi.2020.09.020. Epub 2020 Sep 17.
Vibrio vulnificus is a major pathogen of cultured Cynoglossus semilaevis and results in skin ulceration and haemorrhage, but the proteomic mechanism of skin immunity against V. vulnificus remains unclear. In this study, we investigated the histopathology and skin immune response in C. semilaevis with V. vulnificus infection at the protein levels, the differential proteomic profiling of its skin was examined by using iTRAQ and LC-MS/MS analyses. A total of 951 proteins were identified in skin, in which 134 and 102 DEPs were screened at 12 and 36 hpi, respectively. Selected eleven immune-related DEPs (pvβ, Hsp71, MLC1, F2, α2ML, HCII, C3, C5, C8β, C9 and CD59) were verified for their immune roles in the V. vulnificus infection via using qRT-PCR assay. KEGG enrichment analysis revealed that most of the identified immune proteins were significantly associated with complement and coagulation cascades, antigen processing and presentation, salivary secretion and phagosome pathways. To our knowledge, this study is the first to describe the proteome response of C. semilaevis skin against V. vulnificus infection. The outcome of this study contributed to provide a new perspective for understanding the molecular mechanism of local skin mucosal immunity, and facilitating the development of novel mucosal vaccination strategies in fish.
创伤弧菌是半滑舌鳎养殖的主要病原体，可导致皮肤溃疡和出血，但皮肤对创伤弧菌免疫的蛋白质组机制尚不清楚。本研究在蛋白质水平上研究了半滑舌鳎感染创伤弧菌后的组织病理学和皮肤免疫反应，通过 iTRAQ 和 LC-MS/MS 分析检测了其皮肤的差异蛋白质组谱。在皮肤中鉴定出 951 种蛋白质，在 12 和 36 hpi 时分别筛选出 134 和 102 个差异表达蛋白。通过 qRT-PCR 检测，选择了 11 种免疫相关的差异表达蛋白（pvβ、Hsp71、MLC1、F2、α2ML、HCII、C3、C5、C8β、C9 和 CD59）来验证它们在创伤弧菌感染中的免疫作用。KEGG 富集分析表明，大多数鉴定出的免疫蛋白与补体和凝血级联、抗原加工和呈递、唾液分泌和吞噬体途径显著相关。据我们所知，这项研究首次描述了半滑舌鳎皮肤对创伤弧菌感染的蛋白质组反应。本研究的结果为理解鱼类局部皮肤黏膜免疫的分子机制提供了新的视角，并有助于开发鱼类黏膜疫苗接种的新策略。
