Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, People's Republic of China.
Tianjin Fisheries Research Institute, 422 Jiefang Nan Road, He Xi District, Tianjin, 300221, People's Republic of China.
BMC Microbiol. 2022 Aug 16;22(1):200. doi: 10.1186/s12866-022-02610-1.
Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity.
创伤弧菌是一种致病性细菌，可导致海洋鱼类患病，影响全球水产养殖和人类健康。2021 年 5 月，在渤海湾地区，商业养殖的珍珠龙胆石斑鱼（♀ Epinephelus fuscoguttatus ×♂ Epinephelus lanceolatus）爆发疾病，造成巨大的经济损失。患病鱼出现皮肤损伤、腹部积水，并表现出组织和器官损伤。创伤弧菌生物型 2 已在鳗鱼和其他海洋鱼类中报告，但在珍珠龙胆石斑鱼中报告较少。在本研究中，根据生理生化特征以及 16S rRNA 基因和 gyrB 测序、毒力基因检测和递归感染实验的结果，从患病鱼中分离出的致病菌株被鉴定为创伤弧菌 EPL 0201 生物型 2。为了全面了解该菌株的致病性和耐药性，进行了全基因组测序。全基因组分析表明该菌株的基因图谱完整。毒力因子数据库注释结果表明，该菌株具有引起宿主疾病的关键毒力因子基因 vvhA 和 rtxA。此外，该菌株还具有对抗头孢菌素、氨基糖苷类、四环素类和磺胺类药物的耐药基因。药敏试验证实了基因组中存在这些耐药基因。本研究结果表明，创伤弧菌 EPL 0201 生物型 2 是一种具有高致病性的多药耐药株。
