Terranova V P, Hic S, Franzetti L, Lyall R M, Wikesjö U M
J Periodontol. 1987 Apr;58(4):247-57. doi: 10.1902/jop.1987.58.4.247.
Recent investigations on regeneration of the periodontium have attempted to define factors involved in the formation of a new connective tissue attachment. One essential biological event involved in tissue regeneration is directed cell migration (chemotaxis). Extracellular matrix proteins have been shown to influence chemotaxis, cell proliferation and differentiation. Recently, the extracellular matrix proteins, fibronectin (FN) and laminin (LM), and the polypeptide, endothelial cell growth factor (ECGF), have been shown to stimulate a variety of biological processes. Current assay systems which attempt to define cell migration are the Boyden chamber assay and a random cell migration assay. Neither assay system adequately defines in vivo cell migration. Here we present a new in vitro assay system that tests the capacity of several biological response modifiers applied on dentin to stimulate a chemotactic and proliferative response from various cell types. The assay system consists of two types of assays. Assay I measures the chemotactic activity of test substances bound to dentin. In this assay cells must actively move through a filter (Nuclepore) towards a factor bound to dentin. Assay II examines the ability of dentin-bound biological response modifiers to stimulate directed movement and proliferation of cells on dentin surfaces. We report that periodontal ligament (PDL) cells migrate towards FN and ECGF; that PDL cell migration is enhanced when dentin is preconditioned with tetracycline HCl; that PDL cells have an increased proliferative response when dentin is conditioned with both FN and ECGF; that gingival epithelial cells have increased migratory and proliferative responses when LM is used to condition dentin; and that there is a reciprocal utilization of biological response modifiers by gingival epithelial cells and PDL cells.
近期关于牙周组织再生的研究试图明确参与形成新的结缔组织附着的相关因素。组织再生过程中涉及的一个重要生物学事件是定向细胞迁移（趋化性）。细胞外基质蛋白已被证明会影响趋化性、细胞增殖和分化。最近，细胞外基质蛋白纤连蛋白（FN）和层粘连蛋白（LM）以及多肽内皮细胞生长因子（ECGF）已被证明能刺激多种生物学过程。目前用于确定细胞迁移的检测系统是博伊登小室检测法和随机细胞迁移检测法。但这两种检测系统都无法充分界定体内细胞迁移情况。在此，我们介绍一种新的体外检测系统，该系统可测试应用于牙本质的几种生物反应调节剂刺激多种细胞类型产生趋化和增殖反应的能力。该检测系统由两种检测方法组成。检测方法I测量与牙本质结合的测试物质的趋化活性。在这种检测中，细胞必须主动穿过滤膜（核孔滤膜），朝着与牙本质结合的因子移动。检测方法II检测与牙本质结合的生物反应调节剂刺激细胞在牙本质表面定向移动和增殖的能力。我们报告如下：牙周膜（PDL）细胞向FN和ECGF迁移；当牙本质用盐酸四环素预处理时，PDL细胞迁移增强；当牙本质用FN和ECGF处理时，PDL细胞的增殖反应增强；当用LM处理牙本质时，牙龈上皮细胞的迁移和增殖反应增强；并且牙龈上皮细胞和PDL细胞对生物反应调节剂存在相互利用的情况。
