Nishimura F, Terranova V P
Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, New York, NY 10032, USA.
J Dent Res. 1996 Apr;75(4):986-92. doi: 10.1177/00220345960750041401.
Selective recruitment of periodontal ligament cells to a previously exposed root surface is believed to enhance periodontal regeneration. It has been hypothesized that competition from gingival fibroblasts may reduce the potential of periodontal regeneration. We compared the migratory responses of PDL cells and gingival fibroblasts to a variety of biologicals. Parallel experiments designed to examine the directed migration responses of both periodontal ligament cells (PDL cells) and gingival fibroblasts (GF) isolated from the same donors were conducted using Platelet Derived Growth Factor (PDGF), Insulin Like Growth Factor-I, -II (IGF-I, -II), Epidermal Growth Factor (EGF), Transforming Growth Factor-beta (TGF-beta), and the chemotactic factor derived from the conditioned culture media of PDL cells (termed PDL-CTX) as attractants. Both PDL cells and GF exhibited dose-dependent migratory responses when challenged with PDGF, IGF-I, IGF-II, EGF, and TGF-beta. However, when these cells were challenged with PDL-CTX, only PDL cells migrated in a specific dose-dependent manner, while GF were refractive to PDL-CTX stimulation. Additionally, concentrated conditioned culture media from cultures of gingival fibroblasts did not stimulate PDL cell migratory responses. In other experiments, antibody directed against PDGF, FGF, TGF-beta, IGF-I, IGF-II, NGF, and EGF did not inhibit the PDL-CTX-elicited response in PDL cells. Previous studies have suggested that success of periodontal therapy depends on the specific attachment, migration, and proliferation of selected periodontal ligament cells. The data presented in this manuscript suggest that both PDL cells and gingival fibroblasts respond to a multitude of growth factors. PDL-CTX was found to be PDL-cell-specific for directed migration. Thus, we conclude that any biological therapeutic regime for periodontal regeneration should include PDL-cell-specific agents.
人们认为，选择性地招募牙周膜细胞至先前暴露的牙根表面可促进牙周组织再生。据推测，牙龈成纤维细胞的竞争可能会降低牙周组织再生的潜力。我们比较了牙周膜细胞和牙龈成纤维细胞对多种生物制剂的迁移反应。使用血小板衍生生长因子（PDGF）、胰岛素样生长因子-I、-II（IGF-I、-II）、表皮生长因子（EGF）、转化生长因子-β（TGF-β）以及源自牙周膜细胞条件培养基的趋化因子（称为PDL-CTX）作为趋化剂，进行了平行实验，以检测从相同供体分离的牙周膜细胞（PDL细胞）和牙龈成纤维细胞（GF）的定向迁移反应。当用PDGF、IGF-I、IGF-II、EGF和TGF-β刺激时，PDL细胞和GF均表现出剂量依赖性迁移反应。然而，当用PDL-CTX刺激这些细胞时，只有PDL细胞以特定的剂量依赖性方式迁移，而GF对PDL-CTX刺激无反应。此外，牙龈成纤维细胞培养物的浓缩条件培养基不会刺激PDL细胞的迁移反应。在其他实验中，针对PDGF、FGF、TGF-β、IGF-I IGF-II、NGF和EGF 的抗体不会抑制PDL细胞中PDL-CTX引发的反应。先前的研究表明，牙周治疗的成功取决于所选牙周膜细胞的特异性附着、迁移和增殖。本论文提供的数据表明，PDL细胞和牙龈成纤维细胞均对多种生长因子有反应。发现PDL-CTX对PDL细胞的定向迁移具有特异性。因此，我们得出结论，任何用于牙周组织再生的生物治疗方案都应包括针对PDL细胞的药物。
