Li Zhong-Wen, Zhang Ting-You, Yue Guo-Jun, Tian Xin, Wu Jin-Zhi, Feng Guang-Yong, Wang Yong-Sheng
Department of Thoracic Oncology, State Key Laboratory of Biotherapy and Cancer Centre, West China Hospital, Sichuan University, Chengdu, China.
Institute of Clinical Pharmacology, GCP Center, West China Hospital, Sichuan University, Chengdu, China.
Ann Transl Med. 2020 Aug;8(16):1007. doi: 10.21037/atm-20-5332.
It has been observed that lncRNAs have been taking part in many cancer progressions, including non-small cell lung cancer and gastric cancer. Meanwhile, lncRNA small nucleolar RNA host gene 22 (SNHG22) has been studied, taking part in the progression of ovarian epithelial carcinoma. However, we know little about the function of SNHG22 in esophageal squamous cell carcinoma (ESCC).
In this study, we will explore the inner mechanism of SNHG22 in ESCC. Quantitative real-time PCR (qRT-PCR) assay was implemented in ESCC cells for detecting the expression of lncRNA, SNHG22, and miR-429. Also, functional experiments, including CCK8 and colony formation assay, were implemented to assess the growth of ESCC cells. Meanwhile, flow cytometry analysis was conducted to test the apoptosis of ESCC cells. The immunofluorescence (IF) assay and western blot were conducted to verify the autophagy of ESCC cells.
Inhibition of SNHG22 was found that can inhibit the progression and promotes autophagy and apoptosis of ESCC cells. Meanwhile, as subcellular fraction assay and FISH assay found that SNHG22 mainly in the cytoplasm, miR-429 was found can bind to SNHG22 and SESN3 by RIP assay and luciferase reporter assay. SESN3 was found it can play the oncogene in ESCC cells.
SNHG22 promotes the progression of ESCC by the miR-429/SESN3 axis.
据观察，长链非编码RNA（lncRNAs）参与了许多癌症进程，包括非小细胞肺癌和胃癌。同时，lncRNA小核仁RNA宿主基因22（SNHG22）已被研究，其参与卵巢上皮癌的进程。然而，我们对SNHG22在食管鳞状细胞癌（ESCC）中的功能知之甚少。
在本研究中，我们将探索SNHG22在ESCC中的内在机制。在ESCC细胞中进行定量实时PCR（qRT-PCR）检测，以检测lncRNA、SNHG22和miR-429的表达。此外，进行了包括CCK8和集落形成试验在内的功能实验，以评估ESCC细胞的生长。同时，进行流式细胞术分析以检测ESCC细胞的凋亡。进行免疫荧光（IF）试验和蛋白质印迹法以验证ESCC细胞的自噬。
发现抑制SNHG22可抑制ESCC细胞的进程，并促进其自噬和凋亡。同时，通过亚细胞分级分离试验和荧光原位杂交试验发现SNHG22主要位于细胞质中，通过RNA免疫沉淀试验（RIP）和荧光素酶报告基因试验发现miR-429可与SNHG22和SESN3结合。发现SESN3在ESCC细胞中可发挥癌基因作用。
SNHG22通过miR-429/SESN3轴促进ESCC的进程。
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