Roughneen P T, Didlake R, Kumar S C, Kahan B D, Rowlands B J
J Surg Res. 1987 May;42(5):447-53. doi: 10.1016/0022-4804(87)90017-5.
Recent studies have demonstrated that hepatic dysfunction, induced by experimental biliary ligation (EBL), impairs lymphocytic responsiveness to PHA stimulation in vitro and to cellular antigens in vivo. This suppression appears to be selective for T-cell mechanisms while B-cell-mediated functions remain intact. The purpose of this study was to determine whether coexisting hepatic insufficiency could exert a protective effect on vascularized or nonvascularized allograft survival in the transplanted recipient. Female Wistar-Furth (Rtlw) 225 g rats were assigned randomly to three groups: EBL, sham operation (Sham) and normal control (NC). Fourteen days following operation animals received heterotopic cardiac or skin allografts from Buffalo (Rtlb) donors. Cardiac and skin graft survival was determined daily, rejection was confirmed histologically, and technical failures were omitted from analysis. Allograft survival was expressed as median survival time +/- SEM. Serum total bilirubin (mean +/- SEM) was significantly elevated at Day 14 in EBL animals compared to Sham and NC groups (15.1 +/- 1.0 vs 0.1 +/- 0 and 0.2 +/- 0.1 mg/dl, respectively, P less than 0.01). Median cardiac allograft survival time by Probit was 10.6 +/- 2.6 vs 5.6 +/- 0.7 and 6.0 +/- 0.9 days, respectively (P less than 0.03). Skin graft survival (mean and range) was similar in all groups. These results demonstrate that EBL in the rat suppresses T-cell function and significantly prolongs vascularized allograft survival, but not skin allograft survival across the Rtl histocompatibility barrier. The mechanism whereby coexisting hepatic dysfunction exerts a protective effect on vascularized allograft survival warrants further investigation.
最近的研究表明，实验性胆管结扎（EBL）诱导的肝功能障碍会损害淋巴细胞在体外对PHA刺激以及在体内对细胞抗原的反应性。这种抑制似乎对T细胞机制具有选择性，而B细胞介导的功能则保持完整。本研究的目的是确定并存的肝功能不全是否能对移植受体中血管化或非血管化同种异体移植物的存活发挥保护作用。将体重225 g的雌性Wistar-Furth（Rtlw）大鼠随机分为三组：EBL组、假手术（Sham）组和正常对照（NC）组。术后14天，动物接受来自布法罗（Rtlb）供体的异位心脏或皮肤同种异体移植物。每天测定心脏和皮肤移植物的存活情况，通过组织学确认排斥反应，并将技术失败情况排除在分析之外。同种异体移植物存活情况以中位存活时间±SEM表示。与Sham组和NC组相比，EBL组动物在第14天时血清总胆红素（平均值±SEM）显著升高（分别为15.1±1.0 vs 0.1±0和0.2±0.1 mg/dl，P<0.01）。通过概率单位法计算，心脏同种异体移植物的中位存活时间分别为10.6±2.6天、5.6±0.7天和6.0±0.9天（P<0.03）。所有组的皮肤移植物存活情况（平均值和范围）相似。这些结果表明，大鼠中的EBL会抑制T细胞功能并显著延长血管化同种异体移植物的存活时间，但不会延长跨越Rtl组织相容性屏障的皮肤同种异体移植物的存活时间。并存的肝功能障碍对血管化同种异体移植物存活发挥保护作用的机制值得进一步研究。
