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MTO 和 DMT 佐剂的 CMFO 亚单位疫苗对 感染诱导的免疫原性和保护效力的差异。

Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Infection.

机构信息

Department of Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan, China.

出版信息

J Immunol Res. 2020 Sep 4;2020:2083793. doi: 10.1155/2020/2083793. eCollection 2020.

DOI:10.1155/2020/2083793
PMID:32953889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7487112/
Abstract

Tuberculosis (TB) remains a major and global problem of public health. An effective TB subunit vaccine is urgently needed. Proper selection of the delivery system for the vaccine is crucial for inducing an appropriate immune response tailored to control the target pathogen. In this study, we compared the immunogenicity and protective efficacy of CMFO subunit vaccines against primary progressive TB in two different adjuvant systems: the MTO oil-in-water (O/W) emulsion composed of monophosphoryl lipid A (MPL), trehalose-6,60-dibehenate (TDB), and oil in water emulsion MF59 and the DMT liposome containing dimethyldioctadecylammonium bromide (DDA), monophosphoryl lipid A (MPL), and trehalose-6,60-dibehenate (TDB). Our results demonstrated that the DMT-adjuvanted CMFO could confer more significant protection against infection than the CMFO/MTO did in mice. In particular, the adjuvant DMT showed a stronger ability than the O/W emulsion to adjuvant CMFO subunit vaccine and enhanced protection, attributed to elicit Th1-biased responses, strong Th1/Th17 cytokine responses, and IFN- or IL-2 T cell responses. Therefore, our findings demonstrate that the liposome delivery system shows more effectiveness to adjuvant TB subunit vaccine than O/W emulsion and highlight the importance of adjuvant formulation for the better efficacy of a protein vaccine.

摘要

结核病（TB）仍然是一个主要的全球性公共卫生问题。急需一种有效的结核病亚单位疫苗。疫苗传递系统的正确选择对于诱导针对目标病原体的适当免疫反应至关重要。在这项研究中，我们比较了两种不同佐剂系统中的 CMFO 亚单位疫苗对原发性进行性结核病的免疫原性和保护效力：由单磷酰脂质 A（MPL）、海藻糖-6,60-二硬脂酸酯（TDB）和油包水乳剂 MF59 组成的 MTO 油包水乳剂和含有二甲基二十八烷基溴化铵（DDA）、单磷酰脂质 A（MPL）和海藻糖-6,60-二硬脂酸酯（TDB）的 DMT 脂质体。我们的结果表明，与 CMFO/MTO 相比，DMT 佐剂的 CMFO 能够更有效地预防小鼠感染。特别是，佐剂 DMT 比 O/W 乳液更能增强 CMFO 亚单位疫苗的佐剂作用，增强保护作用，归因于引发 Th1 偏向反应、强烈的 Th1/Th17 细胞因子反应以及 IFN- 或 IL-2 T 细胞反应。因此，我们的研究结果表明，脂质体传递系统对佐剂结核病亚单位疫苗的效果优于 O/W 乳液，并强调了佐剂配方对蛋白质疫苗更好效果的重要性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/11b874ac9e8f/JIR2020-2083793.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/a47f8fcbc5c6/JIR2020-2083793.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/ab3958170c9a/JIR2020-2083793.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/56585cb05e79/JIR2020-2083793.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/11b874ac9e8f/JIR2020-2083793.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/a47f8fcbc5c6/JIR2020-2083793.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/ab3958170c9a/JIR2020-2083793.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/56585cb05e79/JIR2020-2083793.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3458/7487112/11b874ac9e8f/JIR2020-2083793.004.jpg

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