Liang Qian, Wang Xingyu, Yang Shuo, Yu Linhong, Gao Qiaohui, Yang Xingbin, Zhao Yan
Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, China.
Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
J Food Biochem. 2020 Nov;44(11):e13462. doi: 10.1111/jfbc.13462. Epub 2020 Sep 21.
Colorectal cancer, the most common malignancy in Asian and west world, is listed as the fourth lethal neoplastic disease with increasing incidence worldwide. Recently, Ziziphus jujube were reported with hepatoprotective, antihypertensive, and hypoglycemic functions. The polysaccharides from Ziziphus jujube was considered as the main component for these bioactivities. In this study, polysaccharides from Ziziphus jujube cv. Goutouzao (GZSP) was comprehensively investigated, and characterized as a heteropolysaccharide with antioxidant activity. Besides, it can stimulate the viability of immune cells RAW 264.7, which in turn inhibited the proliferation of colorectal carcinoma cells (LoVo) by inducing apoptosis, arresting cell cycle in G0/G1, and increasing intracellular ROS, as demonstrated by Flow Cytometric analyses. The results suggest that, different from chemotherapeutic modalities, GZSP can exert antitumor effects by activating immune reaction, providing more evidence for the development of GZSP-based functional foods and anticancer drugs serving as human colon cancer prevention. PRACTICAL APPLICATIONS: Natural products from medicinal and edible plant are great sources of phytochemicals beneficial to human health, such as tea polyphenols, carotenoids, and phytosterols, etc. In this study, GZSP, the polysaccharides from a well-received fruit, Ziziphus jujube cv. Goutouzao, has been comprehensively investigated. The results show that GZSP fights against free radicals commonly found in human circulation, a property that enables it to be used as an antioxidant food additive with jujube flavor. More importantly, GZSP impedes neoplastic progression by activating immune response, as evidenced by the inhibition of colorectal carcinoma (LoVo) cells. Comparing with chemotherapies usually imposing cytotoxicity on normal tissues, natural product GZSP is able to exert the antiproliferative effects on carcinoma cells with minimal side-effects. Therefore, GZSP-based functional foods and anticancer drugs with the purpose of preventing human colon cancer formation are promising to be developed.
结直肠癌是亚洲和西方世界最常见的恶性肿瘤，被列为全球发病率不断上升的第四大致命性肿瘤疾病。最近，有报道称枣具有保肝、降压和降血糖功能。枣多糖被认为是这些生物活性的主要成分。在本研究中，对狗头枣品种的枣多糖（GZSP）进行了全面研究，并将其表征为具有抗氧化活性的杂多糖。此外，它可以刺激免疫细胞RAW 264.7的活力，进而通过诱导凋亡、使细胞周期停滞在G0/G1期以及增加细胞内活性氧来抑制结肠癌细胞（LoVo）的增殖，流式细胞术分析证明了这一点。结果表明，与化疗方式不同，GZSP可以通过激活免疫反应发挥抗肿瘤作用，为开发基于GZSP的功能性食品和抗癌药物以预防人类结肠癌提供了更多证据。实际应用：药用和食用植物中的天然产物是对人类健康有益的植物化学物质的重要来源，如茶多酚、类胡萝卜素和植物甾醇等。在本研究中，对一种广受欢迎的水果——狗头枣品种的枣多糖GZSP进行了全面研究。结果表明，GZSP能对抗人体循环中常见的自由基，这一特性使其能够用作具有枣味的抗氧化食品添加剂。更重要的是，GZSP通过激活免疫反应来阻碍肿瘤进展，结肠癌细胞（LoVo）的抑制证明了这一点。与通常对正常组织具有细胞毒性的化疗方法相比，天然产物GZSP能够对癌细胞发挥抗增殖作用，且副作用最小。因此，有望开发出以预防人类结肠癌形成为目的的基于GZSP的功能性食品和抗癌药物。
