Tkachenko A G, Chudinov A A
Mikrobiologiia. 1987 Jan-Feb;56(1):58-63.
The quantitative determination of adenyl nucleotides based on the separation of their dansyl derivatives by thin layer chromatography has made it possible to study the dynamics of changes in the pool of ATP, ADP and AMP in Escherichia coli K-12 during its synchronous growth after glucose starvation. The energy parameters (the adenylate pool, energy charge, teh ATP/ADP ratio, the rates of oxygen uptake and ATP generation, the economic coefficients of oxygen and ATP utilization) were compared with changes in the growth characteristics (the rate of growth and biomass concentration). This comparison allowed the authors to draw the conclusion about the uncoupled constructive and energy metabolism and about the possible regulatory role of energy parameters in the synchronised culture growth.
基于丹磺酰衍生物通过薄层色谱法分离对腺苷酸核苷酸进行定量测定，使得研究大肠杆菌K-12在葡萄糖饥饿后同步生长期间ATP、ADP和AMP库中变化的动力学成为可能。将能量参数（腺苷酸库、能荷、ATP/ADP比率、氧气摄取和ATP生成速率、氧气和ATP利用的经济系数）与生长特性（生长速率和生物量浓度）的变化进行了比较。这种比较使作者能够得出关于建设性代谢与能量代谢解偶联以及能量参数在同步培养生长中可能的调节作用的结论。
