Tkachenko A G
Mikrobiologiia. 1988 Jul-Aug;57(4):615-22.
The physiology of Escherichia coli feed batch cultures grown in a mineral medium with glucose was studied by analysing the intracellular content of adenylate nucleotides, the respiration rate, and the calculated economic coefficients of substrate and oxygen utilisation (Yx/s and YO2) which were compared with the growth parameters. The cultures were characterised by a rapid change of the transitional states associated with substrate exhaustion, limited oxygen supply, incomplete growth synchronisation and metabolite poisoning. The transitional states determined the oscillatory mode of the constructive and energetic parameters whose comparison allowed one to reveal the periods of incomplete coupling between reactions which generated and consumed the energy.
通过分析腺苷酸核苷酸的细胞内含量、呼吸速率以及计算得出的底物和氧气利用的经济系数（Yx/s和YO2），并将其与生长参数进行比较，研究了在含有葡萄糖的矿物培养基中生长的大肠杆菌分批补料培养物的生理学特性。这些培养物的特点是与底物耗尽、氧气供应有限、生长同步不完全和代谢物中毒相关的过渡状态迅速变化。过渡状态决定了构建和能量参数的振荡模式，通过比较这些参数可以揭示产生和消耗能量的反应之间不完全耦合的时期。
