Dardick I, George D, Jeans M T, Wittkuhn J F, Skimming L, Rippstein P, van Nostrand A W
Oral Surg Oral Med Oral Pathol. 1987 Mar;63(3):325-34. doi: 10.1016/0030-4220(87)90199-x.
Acinic cell carcinomas, in some instances, contain a component of intercalated duct cells. However, the manner in which this element is integrated within the more obvious acinar cells, as well as the role neoplastic intercalated duct cells play in determining morphologic patterns in acinic cell tumors, has not been fully investigated. Ultrastructural study and immunostaining with antibodies to cytokeratins and to S-100 protein carried out in nine cases of parotid acinic cell carcinoma suggest two basic differentiation patterns. In three cases, the lesions were essentially composed of acinar cells (with variation in the number and form of secretory granules), and one of these tumors was unique in having ultrastructural evidence of differentiated myoepithelial cells. In the second group of six cases, there was light microscopic, ultrastructural, and immunohistochemical evidence of a significant component of intercalated duct cells. By means of both immunostaining (intercalated ducts were positive for keratin and S-100 protein; acinar cells were negative for both antigens) and electron microscopy, flattened-to-cuboidal intercalated duct cells were noted to enclose and, presumably, to be involved in the formation of microcystic spaces. Acinic cell carcinomas with a more solid growth pattern contained groups of intercalated duct cells positive for keratin and S-100 protein. Ultrastructurally, these cells were organized into well-formed ducts related to nests of acinar cells. Acinic cell carcinoma is another class of salivary gland tumor in which there can be an integrated proliferation of intercalated duct and acinar cells and, infrequently, of myoepithelial cells, all organized in a simulation of the intercalated duct-acinar unit of the normal salivary gland.
在某些情况下，腺泡细胞癌含有闰管细胞成分。然而，这种成分整合于更明显的腺泡细胞内的方式，以及肿瘤性闰管细胞在决定腺泡细胞肿瘤形态学模式中所起的作用，尚未得到充分研究。对9例腮腺腺泡细胞癌进行的超微结构研究以及用细胞角蛋白和S-100蛋白抗体进行的免疫染色提示了两种基本的分化模式。在3例中，病变主要由腺泡细胞组成（分泌颗粒的数量和形态有所变化），其中1例肿瘤在超微结构上有分化的肌上皮细胞的证据，这是独特的。在第二组6例中，有光镜、超微结构和免疫组化证据表明存在大量闰管细胞成分。通过免疫染色（闰管对角蛋白和S-100蛋白呈阳性；腺泡细胞对两种抗原均呈阴性）和电子显微镜检查，发现扁平至立方形的闰管细胞包绕并可能参与微囊腔隙的形成。生长模式更实性的腺泡细胞癌含有对角蛋白和S-100蛋白呈阳性的闰管细胞群。在超微结构上，这些细胞组织成与腺泡细胞巢相关的结构良好的导管。腺泡细胞癌是另一类唾液腺肿瘤，其中闰管和腺泡细胞，偶尔还有肌上皮细胞可发生整合性增殖，所有这些细胞都模拟正常唾液腺的闰管-腺泡单位进行组织排列。
