Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
Institute of Food Safety and Health, National Taiwan University, Taipei, Taiwan.
J Ethnopharmacol. 2021 Jan 30;265:113395. doi: 10.1016/j.jep.2020.113395. Epub 2020 Sep 19.
Armillaria mellea (Vahl) P. Kumm. (AM) is an edible mushroom that has been reported as treatment for several neurological disorders, such as dizziness and epilepsy in Asia. Importantly, AM shares a symbiotic relationship with Gastrodia elata Blume (GE), a medicinal herb with antidepressant-like properties. Researchers believe that AM may possess pharmacological properties similar to GE due to their symbiosis, however, few studies have investigated the pharmacological effect of AM.
The aim of this study was to explore the potential of AM as an antidepressant in forced-swimming test (FST) and unpredictable chronic mild stress (UCMS) rodent models and investigate its possible underlying mechanism.
Rats were orally administrated with 250, 500, and 1000 mg/kg body weight (bw) water extract of AM (WAM) for 28 and 35 consecutive days prior to the FST and UCMS protocols, respectively. The cerebral serotonin (5-HT) and the metabolites in the frontal cortex of rats were measured. The brain was dissected and the blood was collected to investigate the levels of inflammatory-related signaling pathway.
All doses of WAM reduced the immobility time in the FST without disturbing autonomic locomotion. All doses of WAM prevented stress-induced abnormal behaviors in the UCMS model, including decreased sucrose preference and hypoactivity. 500 and 1000 mg/kg bw WAM attenuated the stress-induced increases in IL-1β and TNF-α in the serum and cerebrum. 1000 mg/kg bw WAM alleviated brain inflammation by reducing the protein expression of ionized calcium binding adaptor molecule 1.
WAM exhibited acute and chronic antidepressant-like effects, and may result from the anti-inflammatory actions. Therefore, the development of AM as a dietary therapy or adjuvant for depression treatment should be considered.
蜜环菌（Vahl）P. Kumm.（AM）是一种可食用的蘑菇，据报道可用于治疗亚洲的几种神经系统疾病，如头晕和癫痫。重要的是，AM 与天麻（GE）有着共生关系，GE 是一种具有抗抑郁作用的草药。研究人员认为，由于它们的共生关系，AM 可能具有与 GE 相似的药理学特性，但很少有研究调查 AM 的药理学作用。
本研究旨在探讨 AM 在强迫游泳试验（FST）和不可预测的慢性轻度应激（UCMS）啮齿动物模型中作为抗抑郁药的潜力，并研究其可能的潜在机制。
大鼠分别连续 28 天和 35 天口服 250、500 和 1000 mg/kg 体重的 AM 水提取物（WAM），然后进行 FST 和 UCMS 方案。测量大鼠大脑皮质中的 5-羟色胺（5-HT）和代谢物。分离大脑并收集血液以研究炎症相关信号通路的水平。
WAM 的所有剂量均减少了 FST 中的不动时间，而不影响自主运动。WAM 的所有剂量均预防了 UCMS 模型中应激引起的异常行为，包括蔗糖偏好降低和活动减少。500 和 1000 mg/kg bw WAM 减轻了应激引起的血清和大脑中 IL-1β 和 TNF-α 的增加。1000 mg/kg bw WAM 通过降低离子钙结合接头分子 1 的蛋白表达来减轻脑炎症。
WAM 表现出急性和慢性抗抑郁样作用，可能是由于抗炎作用。因此，应考虑将 AM 开发为饮食疗法或抗抑郁治疗的辅助药物。
