Li Hongyu, Xu Guangyu, Yuan Guangxin
School of Pharmacy, Beihua University, Jilin, China.
School of Basic Medicine, Jiamusi University, Jiamusi, China.
Front Pharmacol. 2022 Jul 18;13:919920. doi: 10.3389/fphar.2022.919920. eCollection 2022.
has been known and used in traditional medicine in East Asia for hundreds of years. It has already been reported that extracts have various pharmacological effects, and the polysaccharides of exhibit antioxidant and anti-apoptotic activities. In this study, a water-soluble polysaccharide (AMP-N-a-1), with an average molecular weight of 17 kD, was isolated and purified from the water extract of using DEAE-52, Sepharose CL-4B, and Sephadex G-100 column chromatography. AMP-N-a-1 was mainly composed of Man (1.65%), Glca (1.64%), Rha (1.82%), Gala (2.49%), Glc (90.48%), Gal (0.89%), Xyl (0.42%), and Ara (0.61%). AMP-N-a-1 was used to study the effect on the learning and memory of mice and its underlying mechanisms. The results showed that AMP-N-a-1 could significantly increase the activities of catalase (CAT) and superoxide dismutase (SOD) and reduce the content of nitric oxide (NO) in mouse brain tissue. Meanwhile, AMP-N-a-1 could reduce the contents of norepinephrine (NE) and dopamine (DA) but could increase the content of 5-hydroxytryptamine (5-HT) in mouse brain tissue. In addition, the immunofluorescence experiment showed that AMP-N-a-1 could promote the proliferation of hippocampal dentate gyrus neurons. The above results indicate that AMP-N-a-1 can significantly improve the learning and memory of mice, and the mechanism may be that AMP-N-a-1 can participate in the regulation of learning and memory through a variety of ways.
在东亚传统医学中已为人所知并使用了数百年。已有报道称其提取物具有多种药理作用，且其多糖具有抗氧化和抗凋亡活性。在本研究中，使用DEAE - 52、Sepharose CL - 4B和Sephadex G - 100柱色谱从其水提取物中分离并纯化出一种平均分子量为17 kD的水溶性多糖（AMP - N - a - 1）。AMP - N - a - 1主要由甘露糖（1.65%）、葡萄糖醛酸（1.64%）、鼠李糖（1.82%）、半乳糖醛酸（2.49%）、葡萄糖（90.48%）、半乳糖（0.89%）、木糖（0.42%）和阿拉伯糖（0.61%）组成。使用AMP - N - a - 1研究其对小鼠学习记忆的影响及其潜在机制。结果表明，AMP - N - a - 1可显著提高小鼠脑组织中过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性，并降低一氧化氮（NO）的含量。同时，AMP - N - a - 1可降低小鼠脑组织中去甲肾上腺素（NE）和多巴胺（DA）的含量，但可增加5 - 羟色胺（5 - HT）的含量。此外，免疫荧光实验表明，AMP - N - a - 1可促进海马齿状回神经元的增殖。上述结果表明，AMP - N - a - 1可显著改善小鼠的学习记忆，其机制可能是AMP - N - a - 1可通过多种方式参与学习记忆的调节。
