Hauw J J, de Brunier O, Boutry J M, Schuller E, Lyon-Caen O
Pathol Biol (Paris). 1987 Mar;35(3):319-24.
The myelinotoxicity of sera from multiple sclerosis (MS) patients was determined by the assessment of visible myelin damage in guinea-pig spinal cord-spinal ganglia and in rat cerebella cultivated on collagen-coated coverslips in Leighton tubes. No change was seen in 22 cases. These data show that serum myelinotoxicity is low in MS - as compared with that of Experimental Allergic Encephalomyelitis. It appears a little specific phenomenon, the mechanism of which remains unclear. It can be easily assessed only in very sensitive culture techniques and is best measured by biochemical methods. This does not preclude the pathophysiological significance of the serum myelinotoxicity in MS. Supernatants of cerebro-spinal fluid (CSF) cell cultures in 10 MS patients caused no demyelination. Pooled concentrated supernatants of CSF cell cultures from 13 to 20 MS patients--containing from 7.3 to 11.7 micrograms IgG/ml gave no patent in vitro demyelination in 3 different experiments. The released products of CSF cell cultures in MS are not very toxic for myelin. However these experiments have to be repeated with more sensitive culture techniques, biochemical assay of myelinotoxicity and more concentrated CSF cultures supernatants.
通过评估豚鼠脊髓-脊神经节以及在莱顿管中胶原包被盖玻片上培养的大鼠小脑的可见髓鞘损伤，来测定多发性硬化症（MS）患者血清的髓鞘毒性。22例未见变化。这些数据表明，与实验性变应性脑脊髓炎相比，MS患者血清的髓鞘毒性较低。这似乎是一种有点特殊的现象，其机制尚不清楚。只有在非常敏感的培养技术中才能容易地评估它，最好通过生化方法进行测量。这并不排除血清髓鞘毒性在MS中的病理生理意义。10例MS患者脑脊液（CSF）细胞培养上清液未引起脱髓鞘。来自13至20例MS患者的CSF细胞培养物的合并浓缩上清液（IgG含量为7.3至11.7微克/毫升）在3个不同实验中均未出现明显的体外脱髓鞘现象。MS患者CSF细胞培养物释放的产物对髓鞘的毒性不大。然而，这些实验必须采用更敏感的培养技术、髓鞘毒性的生化检测方法以及更浓缩的CSF培养物上清液重复进行。
