Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea.
Marine Science Institute, Jeju National University, Jeju 63333, Korea.
Mar Drugs. 2020 Sep 17;18(9):470. doi: 10.3390/md18090470.
Diphlorethohydroxycarmalol (DPHC) isolated from (IO) showed potential whitening effects against UV-B radiation. However, the components of IO as well as their molecular mechanism against α-melanocyte-stimulating hormone (α-MSH) have not yet been investigated. Thus, this study aimed to investigate the inhibitory effects of Ishophloroglucin A (IPA), a phlorotannin isolated from brown algae IO, and its crude extract (IOE), in melanogenesis in vivo in an α-MSH-induced zebrafish model and in B16F10 melanoma cells in vitro. Molecular docking studies of the phlorotannins were carried out to determine their inhibitory effects and to elucidate their mode of interaction with tyrosinase, a glycoprotein related to melanogenesis. In addition, morphological changes and melanin content decreased in the α-MSH-induced zebrafish model after IPA and IOE treatment. Furthermore, Western blotting results revealed that IPA upregulated the extracellular related protein expression in α-MSH-stimulated B16F10 cells. Hence, these results suggest that IPA isolated from IOE has a potential for use in the pharmaceutical and cosmetic industries.
二氢愈创木酸（DPHC）从 （IO）中分离出来，具有抗 UV-B 辐射的潜在美白作用。然而，IO 的成分及其对α-促黑色素细胞激素（α-MSH）的分子机制尚未得到研究。因此，本研究旨在探讨从褐藻 IO 中分离出的 Phloroglucin A（IPA）及其粗提物（IOE）对α-MSH 诱导的斑马鱼模型中黑色素生成的体内抑制作用以及在 B16F10 黑素瘤细胞中的体外抑制作用。对 Phlorotannins 进行了分子对接研究，以确定它们的抑制作用，并阐明它们与与黑色素生成有关的糖蛋白酪氨酸酶的相互作用方式。此外，在 IPA 和 IOE 处理后，α-MSH 诱导的斑马鱼模型中的形态变化和黑色素含量降低。此外，Western blot 结果表明，IPA 上调了 α-MSH 刺激的 B16F10 细胞中细胞外相关蛋白的表达。因此，这些结果表明，IOE 中分离出的 IPA 具有在制药和化妆品行业应用的潜力。
