利用脉冲场凝胶电泳技术对粟酒裂殖酵母进行的电泳核型分析。
An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.
作者信息
Smith C L, Matsumoto T, Niwa O, Klco S, Fan J B, Yanagida M, Cantor C R
出版信息
Nucleic Acids Res. 1987 Jun 11;15(11):4481-9. doi: 10.1093/nar/15.11.4481.
Abstract
The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs.
摘要
粟酒裂殖酵母的三条染色体DNA已通过脉冲场凝胶电泳进行了分离。由此产生的分子核型将大大加快该生物体中的基因定位，并且表明该技术的分离范围可扩展到长达900万个碱基对的DNA分子。
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