Li Yi, Yuan Qing, Huang Guoping, Ye Yingchun, Yu Hong, Xie Xiang, Nian Siji
Immunology Laboratory, Basic Medical College, Southwest Medical University, Luzhou 646000, China.
Immunology Laboratory, Basic Medical College, Southwest Medical University, Luzhou 646000, China. *Corresponding author, E-mail:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Aug;36(8):687-692.
Objective To establish a mouse neutrophil-dominated asthma model using house dust mite (HDM). Methods With a random number table, a total of 16 specific pathogen-free (SPF) BALB/c mice were divided into control group and model group. The model group was sensitized and stimulated by HDM nasal drip, and the control group mice were given the same amount of saline. In 24 hours after the last stimulation, the enhanced pause (Penh) was measured by the BUXCO noninvasive lung function detector to determine the airway resistance; the bronchoalveolar lavage fluid (BALF) was collected to count cells and classify inflammatory cells. The infiltration degree of inflammatory cells in the lung tissue was observed by HE staining. The expression of IL-6, IL-17F and IL-1β mRNA was detected by real-time quantitative PCR, and the levels of IL-4, IL-6, IL-17F and tumor necrosis factor α (TNF-α) in BALF were detected by cytometric bead array (CBA). Results Compared with the control group, the value of Penh in the model group was significantly higher; the tracheal and paravascular inflammation significantly aggravated; the levels of IL-6, IL-17F, IL-1β mRNA, the cell count and neutrophil count in BALF, and the levels of neutrophil cytokines IL-6, IL-17F and TNF-α in the BALF increased significantly. Conclusion The mouse neutrophil-dominated house dust mite allergic asthma model has been successfully established.
目的 利用屋尘螨（HDM）建立以小鼠中性粒细胞为主的哮喘模型。方法 采用随机数字表法将16只无特定病原体（SPF）级BALB/c小鼠分为对照组和模型组。模型组采用HDM滴鼻进行致敏和激发，对照组小鼠给予等量生理盐水。末次激发后24小时，用BUXCO无创肺功能检测仪检测增强暂停（Penh）以测定气道阻力；收集支气管肺泡灌洗液（BALF）进行细胞计数和炎性细胞分类。通过HE染色观察肺组织中炎性细胞的浸润程度。采用实时定量PCR检测IL-6、IL-17F和IL-1β mRNA的表达，采用细胞计数珠阵列（CBA）检测BALF中IL-4、IL-6、IL-17F和肿瘤坏死因子α（TNF-α）的水平。结果 与对照组相比，模型组Penh值显著升高；气管及血管周围炎症明显加重；BALF中IL-6、IL-17F、IL-1β mRNA水平、细胞计数及中性粒细胞计数，以及BALF中中性粒细胞细胞因子IL-6、IL-17F和TNF-α水平均显著升高。结论 成功建立了以小鼠中性粒细胞为主的屋尘螨过敏性哮喘模型。
