Department of Anatomy, College of Health Sciences, Osun State University, Osogbo, Osun State, Nigeria).
Department of Anatomy, College of Health Sciences, University of Ilorin, Kwara State, Nigeria.
JBRA Assist Reprod. 2021 Feb 2;25(1):97-103. doi: 10.5935/1518-0557.20200058.
The literature has shown that synthetic antipsychotic drugs induce reproductive toxicity, while psychiatric patients treated with traditionally used antipsychotic herbs (Rauwolfia vomitoria) showed no traces of reproductive toxicity. Thus, this study aimed to investigate the expression of CREM, PRM I and II genes in the testes of Wistar rats treated with antipsychotic drugs: chlorpromazine, Rauwolfia vomitoria (RV) and co-administration of reserpine, zinc and ascorbate (RAZ).
Forty-five adult male Wistar rats with rats with average weight of 180±4.67g were divided into nine groups (A-I) (n=5). Group A was administered saline (control) while rats in Groups B and C received 10 and 20mg/kg body weight (bwt) of chlorpromazine respectively. Groups D and E received 2.5 and 5mg/kg bwt of reserpine, respectively; while Groups F and G received 150 and 300mg/kg bwt of RV leaf extract. Groups H and I received (2.5+5+100) mg/kg bwt and (5+10+200) mg/kg of combination of RAZ, respectively for 56 days.
The CREM, PRM I and II genes were significantly downregulated while significant decreased in serum FSH and testosterone concentration were found in the Chlorpromazine- and Reserpine-treated groups. Groups H and I showed a highly significant upregulation of the CREM, PRM I and II genes, and a highly significant increase in serum FSH and testosterone concentrations.
The study concluded that the HPT-Axis was impaired by chlorpromazine and reserpine, while RV and a combination of RAZ administration enhanced the axis in an animal model. The study recommended that synthetic antipsychotic drugs should be taken with Zinc and Ascorbate in order to help prevent reproductive toxicity associated with antipsychotic drugs. We need further studies in humans to confirm these findings.
文献表明，合成抗精神病药物会导致生殖毒性，而接受传统使用的抗精神病草药（萝芙木）治疗的精神病人则没有生殖毒性的痕迹。因此，本研究旨在研究抗精神病药物：氯丙嗪、萝芙木（RV）和利血平、锌和抗坏血酸联合给药对 Wistar 大鼠睾丸中 CREM、PRM I 和 II 基因的表达。
将 45 只平均体重为 180±4.67g 的成年雄性 Wistar 大鼠分为 9 组（A-I）（n=5）。组 A 给予生理盐水（对照），组 B 和 C 分别给予 10 和 20mg/kg 体重的氯丙嗪。组 D 和 E 分别给予 2.5 和 5mg/kg 体重的利血平；组 F 和 G 分别给予 150 和 300mg/kg 体重的萝芙木叶提取物。组 H 和 I 分别给予（2.5+5+100）mg/kg 体重和（5+10+200）mg/kg 体重的 RAZ 组合，共 56 天。
氯丙嗪和利血平处理组 CREM、PRM I 和 II 基因显著下调，血清 FSH 和睾酮浓度显著降低。组 H 和 I 显示 CREM、PRM I 和 II 基因显著上调，血清 FSH 和睾酮浓度显著升高。
该研究得出结论，氯丙嗪和利血平损害了 HPT 轴，而 RV 和 RAZ 联合给药增强了动物模型中的该轴。该研究建议在服用合成抗精神病药物时应同时服用锌和抗坏血酸，以帮助预防与抗精神病药物相关的生殖毒性。我们需要在人类中进行进一步的研究来证实这些发现。
