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萜类化合物介导的植物-草食动物相互作用特征:静态顶空固相微萃取-气相色谱/四极杆飞行时间质谱筛选。

Profile of Terpenoid Compounds Mediating a Plant-Herbivore Interaction: Screening by Static Headspace Solid-Phase Microextraction-Gas Chromatography/Q-ToF Mass Spectrometry.

机构信息

Centro de Química and Centro de Agroecología, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Av. San Claudio s/n, Ciudad Universitaria, Edif. IC7, Puebla, Pue., 72570, Mexico.

Instituto Tecnológico de Aguascalientes, Av., Adolfo López Mateos #1801 Ote. Bona Gens, Aguascalientes, Ags., 20256, México.

出版信息

Chem Biodivers. 2020 Nov;17(11):e2000564. doi: 10.1002/cbdv.202000564. Epub 2020 Nov 2.

DOI:10.1002/cbdv.202000564
PMID:32961007
Abstract

Conotrachelus dimidiatus (Coleoptera: Curculionidae) interacts with immature guava fruits (Psidium guajava L.) for feed, sleep, mate and oviposit. Determination of the volatile organic compounds (VOCs) emitted by the insect and immature fruits can help improve understanding of plant-insect and intraspecific insect interactions between females and males of C. dimidiatus. Daytime fruit setting emissions of immature guava consist mainly of limonene, caryophyllene, and aromadendrene. In addition to the host's volatiles, the aim of this study was to assess the VOCs released by the insect. Static headspace-solid phase microextraction (SHS-SPME), combined with gas chromatography/quadrupole time of flight mass spectrometry (GC/Q-ToF-MS), allowed the identification of the C terpenoids: grandlure I, II, II, IV, grandisoic acid, papayanol and papayanal bioactive compounds released by female and male C. dimidiatus under laboratory conditions. These chemical compounds are candidates for the preparation of a lure formulation.

摘要

锥卷象(鞘翅目:象甲科)以番石榴幼果(番石榴 Psidium guajava L.)为食、栖息、交配和产卵。确定昆虫和未成熟果实释放的挥发性有机化合物(VOCs)有助于提高对植物-昆虫和雌雄锥卷象种内相互作用的理解。白天,番石榴幼果的排放物主要由柠檬烯、丁香烯和芳樟醇组成。除了寄主挥发物外,本研究还旨在评估昆虫释放的 VOCs。静态顶空固相微萃取(SHS-SPME)结合气相色谱/四极杆飞行时间质谱(GC/Q-ToF-MS),可鉴定出实验室条件下雌、雄锥卷象释放的 C 萜类化合物:大引诱素 I、II、II、IV、大果酸、木瓜醇和木瓜醛生物活性化合物。这些化学化合物是制备引诱剂配方的候选物。

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