Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, São Paulo, Brazil.
National Institute of Science and Technology for Dairy Production Chain (INCT-LEITE), Universidade Estadual de Londrina, Rodovia Celso Garcia Cid-Campus Universitário, PO Box 10011, Londrina, Paraná, 86057-970, Brazil; Laboratório de Reprodução Animal, DCV-CCA-UEL, Londrina, Paraná, Brazil.
Anim Reprod Sci. 2020 Oct;221:106601. doi: 10.1016/j.anireprosci.2020.106601. Epub 2020 Sep 14.
In the present study, there was an evaluation of in vitro embryo production (IVEP) in Bos indicus donor cows with small or large antral follicle counts (AFCs) when there was synchronization of follicular dynamics among cows before ovum pick-up (OPU). Donor cows classified as having small or large AFC were submitted to OPU/IVEP program (Experiment-I) or had follicular-stage synchronization imposed before OPU/IVEP (Experiment-II). In Experiment-I, the cows with a large AFC had a greater (P < 0.01) mean of embryos developing to the blastocyst stage compared to those with a small AFC. In Experiment-II, percentage of viable oocytes/OPU were not affected (P = 0.33) by synchronization of follicular dynamics, but the AFC had an effect (P < 0.0001). There was an interaction (P = 0.01) indicating the larger AFC, with or without imposing of a synchronization treatment regimen, resulted in the most desirable outcome. The number of embryos was affected (P < 0.001) by follicular-stage synchronization and AFC, with there being an interaction (P = 0.002) with the most desirable results for the large AFC-synchronized group. Number of pregnancies was greater (P ≤ 0.02) for recipient females with embryos from synchronized donors and with a large AFC. There was an interaction (P = 0.03) with there being a greater pregnancy percentage for cows with synchronized follicular stages and the large AFC. Bos indicus donor with a large AFC when associated with the synchronization of stage of follicular dynamics pre-OPU results in improvement of the efficacy of IVEP.
在本研究中，我们评估了在卵母细胞采集（OPU）前同步卵泡动力学时，小或大卵泡计数（AFC）的印度野牛供体牛的体外胚胎生产（IVEP）。被分类为小或大 AFC 的供体牛被提交进行 OPU/IVEP 计划（实验-I）或在 OPU/IVEP 之前进行卵泡期同步化（实验-II）。在实验-I 中，大 AFC 的牛胚胎发育到囊胚阶段的平均（P < 0.01）显著高于小 AFC 的牛。在实验-II 中，卵母细胞/OPU 的存活率不受卵泡动力学同步的影响（P = 0.33），但 AFC 有影响（P < 0.0001）。存在交互作用（P = 0.01）表明，大 AFC，无论是否施加同步处理方案，都会产生最理想的结果。胚胎数量受卵泡期同步化和 AFC 的影响（P < 0.001），存在交互作用（P = 0.002），最理想的结果是大 AFC 同步组。胚胎来自同步供体的受体雌性的妊娠率更高（P ≤ 0.02），并且 AFC 较大。存在交互作用（P = 0.03），卵泡阶段同步化和大 AFC 的牛妊娠百分比更高。在 OPU 前同步卵泡动力学阶段时，具有大 AFC 的印度野牛供体可提高 IVEP 的效果。
