病毒宏基因组学揭示了血液系统疾病患者骨髓标本中的多种微小病毒。

Viral metagenomics reveals diverse anelloviruses in bone marrow specimens from hematologic patients.

机构信息

Department of Microbiology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.

Department of Clinical Laboratory, Huai'an Hospital, Xuzhou Medical University, Huai'an 223002, China.

出版信息

J Clin Virol. 2020 Nov;132:104643. doi: 10.1016/j.jcv.2020.104643. Epub 2020 Sep 16.

DOI:10.1016/j.jcv.2020.104643

PMID:32961430

Abstract

BACKGROUND

An infectious etiology has been proposed for many human cancers, but rarely have specific agents been identified. Viral metagenomic technique is useful for identification of viral pathogens potentially existing in bone marrow specimens from hematologic patients.

METHODS

A total of 24 patients were included in this study, including 14 female (58.3 %) and 10 male patients (41.7 %) with a mean age of 55.20 ± 18.02 years (16-89 years).Twenty-four bone marrow specimens were collected from 24 hematologic patients (diagnosed with hypoferric anemia, diffuse large B cell lymphoma, myelodysplastic syndrome, acute myelo-monocytic leukemia, acute myelocytic leukemia with maturation, multiple myeloma, lymphoma angioimmunoblastic T cell, acute myeloid leukemia-M1, polycythemia vera/hypoferric anemia, leukocythemia, or megaloblastic anemia). Viral nucleic acid from marrow samples of hematologic patients were subjected to viral metagenomic analysis. PCR method was used to investigate the prevalence of these new viruses in this cohort of hematologic patients. Phylogenetic tree was established to elucidate the relationship of anelloviruses found here and the previously define ones.

RESULTS

Anelloviridae family are the main group of viruses detected in all the 4 libraries. Forty-six different species of Anelloviruses belonging to genera Alphatorquevirus, Betatorquevirus and Gammatorquevirus and unclassified anellovirus were recovered. Fifteen novel strains with complete ORF1 coding sequence were acquired and phylogenetically analyzed, indicating 8 of the 15 strains are proposed novel species belonging to genus Gammatorquevirus. Nested-PCR were then performed for these15 novel anellovirus strains in the 24 individual bone marrow samples, which showed 13 of them were present in more than one bone marrow samples.

CONCLUSIONS

Diverse types of anellovirus were present in bone marrow samples of hematologic patients. Whether these novel anelloviruses have association with certain hematonosis needs further investigation.

摘要

背景

已经提出了许多人类癌症的传染性病因，但很少有特定的病原体被发现。病毒宏基因组技术可用于鉴定潜在存在于血液系统疾病患者骨髓标本中的病毒病原体。

方法

本研究共纳入 24 例患者，其中女性 14 例（58.3%），男性 10 例（41.7%），平均年龄 55.20±18.02 岁（16-89 岁）。从 24 例血液系统疾病患者的 24 例骨髓标本中采集标本（诊断为低铁性贫血、弥漫性大 B 细胞淋巴瘤、骨髓增生异常综合征、急性髓单核细胞白血病、成熟型急性髓细胞性白血病、多发性骨髓瘤、淋巴瘤血管免疫母细胞 T 细胞、急性髓细胞性白血病-M1、真性红细胞增多症/低铁性贫血、白细胞增多症或巨幼细胞性贫血）。对血液系统疾病患者骨髓样本中的病毒核酸进行病毒宏基因组分析。采用 PCR 方法检测该血液系统疾病患者队列中新病毒的流行情况。建立系统发育树阐明本研究中发现的细小病毒与先前定义的细小病毒之间的关系。

结果

在所有 4 个文库中，细小病毒科是主要的病毒群。共回收 46 种不同种属的细小病毒，属于 Alphatorquevirus、Betatorquevirus 和 Gammatorquevirus 属和未分类细小病毒。获得了 15 个具有完整 ORF1 编码序列的新毒株，并进行了系统发育分析，表明其中 8 株属于 Gammatorquevirus 属的新种。然后对 24 例个体骨髓样本中的这 15 种新型细小病毒株进行巢式 PCR，结果显示其中 13 种存在于多种骨髓样本中。