大孔树脂分离甘草酸及其衍生物水解产物。
Separation of Glycyrrhizic Acid and Its Derivants from Hydrolyzation in Subcritical Water by Macroporous Resin.
机构信息
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
出版信息
Molecules. 2020 Sep 19;25(18):4305. doi: 10.3390/molecules25184305.
Glycyrrhizic acid (GL) and its derivants, glycyrrhetinic acid 3--mono-β-d-glucuronide (GAMG) and glycyrrhetinic acid (GA) hydrolyzed in subcritical water, are bioactive substances and edulcorators. In this work, a separation strategy for these three substances was established. The effects of adsorbent and eluent were investigated by static/dynamic adsorption and multi-stage desorption with the mechanism analysis. The adsorption of them onto EXA50 resin was well fitted by the pseudo second-order kinetic model. The optimal dynamic adsorption flow rate was 6 bed volume (BV)/h, and water of pH = 12 was used to elute GL at 4 BV/h, then -buthanol was used subsequently to elute GA at 1 BV/h, and finally 90% ethanol was applied to elute GAMG at 2 BV/h. As a result, purities of these compounds increased, which demonstrated that this adsorption-desorption technology was simple and efficient, and indicated the potential for large-scale purification and preparation of GL and its derivants in the future.
甘草酸（GL）及其衍生物，即甘草次酸 3--单--β--D--葡萄糖醛酸苷（GAMG）和甘草次酸（GA），在亚临界水中水解后是具有生物活性的物质和甜味剂。在这项工作中，建立了一种分离这三种物质的策略。通过静态/动态吸附和多级解吸，结合机理分析，考察了吸附剂和洗脱剂的影响。它们在 EXA50 树脂上的吸附很好地符合拟二级动力学模型。最佳的动态吸附流速为 6 床体积（BV）/h，用 pH = 12 的水以 4 BV/h 的流速洗脱 GL，然后用正丁醇以 1 BV/h 的流速洗脱 GA，最后用 90%乙醇以 2 BV/h 的流速洗脱 GAMG。结果表明，这些化合物的纯度提高，这表明这种吸附-解吸技术简单高效，为未来 GL 及其衍生物的大规模纯化和制备提供了可能。
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