Beukes Lorika S, Schmidt Stefan
Discipline of Microbiology, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa.
Int J Environ Health Res. 2022 May;32(5):1043-1054. doi: 10.1080/09603123.2020.1823334. Epub 2020 Sep 23.
The presence of and spp. was determined on the skin, personal protective equipment, the municipal vehicle, and various surfaces at ten households in a peri-urban community (KwaZulu-Natal, South Africa) before and after manual emptying of ventilated improved pit latrines. Surface samples (n = 14) were collected using sterile wet wipes, and target bacteria were detected using standard procedures. Additionally, was enumerated in soil samples from an area of open defecation (log 3.7 MPN/g) and areas where geophagia occurred (log 2.7 - log 3.3 MPN/g), using a most probable number (MPN) method. The detection frequency for the target bacteria on household surfaces (e.g., the walkway between the pit latrine and the municipal vehicle) and on municipal workers' hands (which were frequently contaminated before pit emptying), occasionally increased after the pits were emptied, indicating that manual pit emptying might pose a potential health risk to workers and community members.
在南非夸祖鲁 - 纳塔尔省一个城郊社区的十户家庭中，对通风改良坑式厕所进行人工清空前后，在皮肤、个人防护装备、市政车辆以及各个表面上检测了[具体细菌名称1]和[具体细菌名称2]的存在情况。使用无菌湿纸巾采集表面样本（n = 14），并采用标准程序检测目标细菌。此外，使用最大可能数（MPN）法对露天排便区域（对数3.7 MPN/g）和发生食土现象区域（对数2.7 - 对数3.3 MPN/g）的土壤样本中的[具体细菌名称3]进行计数。家庭表面（如坑式厕所与市政车辆之间的通道）和市政工人手上（在清空粪池前经常被污染）目标细菌的检出频率，在粪池清空后偶尔会增加，这表明人工清空粪池可能会对工人和社区成员构成潜在健康风险。
