Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, 305-8569, Japan.
Molecular Imaging Program at Stanford, Bio-X Program, Stanford University School of Medicine, Palo Alto, California, 94304, United States.
Anal Sci. 2021 Feb 10;37(2):233-247. doi: 10.2116/analsci.20R003. Epub 2020 Sep 18.
Bioluminescence (BL) is an excellent optical readout platform that has great potential to be utilized in various bioassays and molecular imaging. The advantages of BL-based bioassays include the long dynamic range, minimal background, high signal-to-noise ratios, biocompatibility for use in cell-based assays, no need of external light source for excitation, simplicity in the measurement system, and versatility in the assay design. The recent intensive research in BL has greatly diversified the available luciferase-luciferin systems in the bioassay toolbox. However, the wide variety does not promise their successful utilization in various bioassays as new tools. This is mainly due to complexity and confusion with the diversity, and the unavailability of defined standards. This review is intended to provide an overview of recent basic developments and applications in BL studies, and showcases the bioanalytical utilities. We hope that this review can be used as an instant reference on BL and provides useful guidance for readers in narrowing down their potential options in their own assay designs.
生物发光（BL）是一种出色的光学读出平台，具有在各种生物测定和分子成像中得到广泛应用的巨大潜力。基于 BL 的生物测定法的优点包括：动态范围长、背景最小、信噪比高、适用于基于细胞的测定法的生物相容性、无需外部光源激发、测量系统简单以及测定设计的多功能性。BL 的最近的密集研究极大地丰富了生物测定工具箱中可用的荧光素酶-荧光素系统。但是，广泛的多样性并不能保证它们作为新工具在各种生物测定法中成功应用。这主要是由于多样性的复杂性和混乱性，以及缺乏明确的标准。本综述旨在提供 BL 研究的最新基础发展和应用的概述，并展示其生物分析效用。我们希望本综述可用作 BL 的即时参考，并为读者在缩小自己的测定设计中的潜在选择提供有用的指导。
