Wadgymar A, Halloran P F
Transplantation. 1987 Jun;43(6):903-8.
During studies to investigate the localization of Ia antigens in normal mouse kidney, a monoclonal antibody (Mab) specific for I-Ab by microcytotoxicity criteria was found to crossreact with a tissue antigen present in the glomeruli and vasculature of murine tissues, irrespective of their Ia haplotype, as well as in human kidneys. Immunofluorescence and immunoperoxidase studies of frozen tissue sections revealed that the antigen responsible for the crossreaction was present in the cytoplasm of glomerular epithelial cells and of smooth muscle of blood vessels. The immunofluorescence pattern of primary cultures of human glomerular epithelial cells and of a glioblastoma cell line indicated that the crossreactive antigen forms part of the cytoskeleton, and resembled the pattern observed with a monoclonal against vimentin, one of the intermediate filaments. Thus this Mab, which is directed against mouse alloantigen Ia.20 in microcytotoxicity was found to crossreact with a cytoskeletal component present in the mouse, as well as in human glomerular epithelial and smooth muscle cells.
在研究正常小鼠肾脏中Ia抗原的定位时，发现一种通过微细胞毒性标准对I-Ab具有特异性的单克隆抗体（Mab）与鼠组织的肾小球和脉管系统中存在的一种组织抗原发生交叉反应，无论其Ia单倍型如何，在人类肾脏中也会发生交叉反应。对冷冻组织切片进行的免疫荧光和免疫过氧化物酶研究表明，负责交叉反应的抗原存在于肾小球上皮细胞和血管平滑肌的细胞质中。人肾小球上皮细胞原代培养物和胶质母细胞瘤细胞系的免疫荧光模式表明，交叉反应性抗原是细胞骨架的一部分，并且类似于用针对波形蛋白（一种中间丝）的单克隆抗体观察到的模式。因此，这种在微细胞毒性中针对小鼠同种异体抗原Ia.20的Mab被发现与小鼠以及人肾小球上皮和平滑肌细胞中存在的一种细胞骨架成分发生交叉反应。
