Linder E, Helin H, Rantala I
Clin Immunol Immunopathol. 1986 Aug;40(2):265-75. doi: 10.1016/0090-1229(86)90030-9.
Glomerular visceral epithelial cells, podocytes, have been shown by immunofluorescence and immunoperoxidase staining methods to bind serum complement (C) components in vitro. Binding of Clq, C4, and C3 was demonstrated and the C4 and C3 binding could be inhibited by EDTA. Thus complement binding had the capacity to cause antibody-independent activation of the "classical" C pathway. The C-binding structures had the same distribution as intermediate filaments (IMFs) of vimentin but not desmin or keratin types. By ultrastructural immunohistochemical staining C3 was observed in association with cytoskeletal IMFs.
肾小球脏层上皮细胞，即足细胞，通过免疫荧光和免疫过氧化物酶染色方法已证实在体外能结合血清补体（C）成分。已证实Clq、C4和C3的结合，且C4和C3的结合可被EDTA抑制。因此，补体结合有能力引发“经典”C途径的抗体非依赖性激活。C结合结构与波形蛋白的中间丝（IMF）分布相同，但与结蛋白或角蛋白类型不同。通过超微结构免疫组织化学染色观察到C3与细胞骨架IMF相关联。
