Mombeini Tajmah, Asadpour Behzadi Babak, Ejtemaei Ramtin, Tahmasbi Freidoun, Kamalinejad Mohammad, Dehpour Ahmad Reza
Department of Pharmacology, School of Medicine, Shahed University, Tehran, Iran.
Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Basic Clin Neurosci. 2020 May-Jun;11(3):369-377. doi: 10.32598/bcn.11.2.2064.1. Epub 2020 May 1.
This study was designed to investigate the possible anticonvulsant effect of acute administration of an aqueous extract of flowers of Alcea aucheri (EFA) in two in vivo seizure models.
Seizures were induced in male adult Swiss mice by administration of Pentylenetetrazol (PTZ) or Maximal Electroshock (MES). Mice were randomly subjected to receive saline, EFA (8.75-175 mg.kg), or diazepam intraperitoneally (i.p.) 15 or 30 min before PTZ injection. In another experiment, mice were treated (i.p.) with saline, EFA (8.75-350 mg.kg), or phenytoin 15 or 30 min before the MES test. Diazepam and phenytoin were used as reference drugs.
EFA (175 mg.kg) significantly increased the PTZ-induced seizure threshold compared with the saline control group 15 min after its administration. In the MES test, the extract (35 mg.kg) increased the latency to onset of tonic Hind Limb Extension (HLE) (seizure activity) compared with the saline group 15 min after treatment. Also, 30 min after treatment, EFA (35, 70, and 175 mg.kg) increased the latency to onset of the seizure, decreased the duration of the seizure (70 mg.kg), and decreased seizure occurrence (350 mg.kg) compared with those of the saline group. At both time points, the extract at all doses significantly reduced the mortality rate compared with the saline group.
These findings provide evidence of a possible anticonvulsant effect of A. aucheri in PTZ and MES seizure models in mice.
本研究旨在探讨急性给予阿尔察花葵（Alcea aucheri）花的水提取物（EFA）在两种体内癫痫模型中可能的抗惊厥作用。
通过给予戊四氮（PTZ）或最大电休克（MES）诱导成年雄性瑞士小鼠癫痫发作。在PTZ注射前15或30分钟，小鼠被随机腹腔注射生理盐水、EFA（8.75 - 175毫克/千克）或地西泮。在另一项实验中，在MES试验前15或30分钟，小鼠腹腔注射生理盐水、EFA（8.75 - 350毫克/千克）或苯妥英。地西泮和苯妥英用作参考药物。
与生理盐水对照组相比，给予EFA（175毫克/千克）后15分钟，PTZ诱导的癫痫阈值显著提高。在MES试验中，与生理盐水组相比，给予提取物（35毫克/千克）后15分钟，强直后肢伸展（HLE，癫痫活动）发作的潜伏期延长。此外，治疗后30分钟，与生理盐水组相比，EFA（35、70和175毫克/千克）增加了癫痫发作的潜伏期，缩短了癫痫发作持续时间（70毫克/千克），并减少了癫痫发作的发生率（350毫克/千克）。在两个时间点，与生理盐水组相比，所有剂量的提取物均显著降低了死亡率。
这些发现提供了证据，表明阿尔察花葵在小鼠PTZ和MES癫痫模型中可能具有抗惊厥作用。
