茎提取物对癫痫急性和慢性实验模型的抗惊厥及抗氧化作用
Anticonvulsant and Antioxidant Effects of Stem Extract on Acute and Chronic Experimental Models of Epilepsy.
作者信息
Reddy Aditya J, Dubey Ashok Kumar, Handu Shailendra S, Sharma Prashant, Mediratta Pramod K, Ahmed Qazi Mushtaq, Jain Seema
机构信息
Department of Pharmacology, SMS and R, Sharda University, Greater Noida, Uttar Pradesh, India.
Department of Pharmacology, UCMS, New Delhi, India.
出版信息
Pharmacognosy Res. 2018 Jan-Mar;10(1):49-54. doi: 10.4103/pr.pr_31_17.
BACKGROUND
(banana) plant extract has been shown to possess antioxidant activity in previous studies. Neuronal injury resulting from oxidative stress is an important factor involved in pathogenesis of epilepsy.
OBJECTIVE
The present study aimed to evaluate the anticonvulsant activity of stem extract (MSSE) in acute and chronic experimental models in mice and its effects on various markers of oxidative stress in the brain of pentylenetetrazole (PTZ)-kindled animals.
MATERIAL AND METHODS
Maximal electroshock seizures (MES) and PTZ-induced convulsion models were used for acute studies. For the chronic study, the effect of MSSE on the development of kindling was studied. For the evaluation of the effects of MSSE on oxidative stress in brain, malondialdehyde (MDA) and reduced glutathione (GSH) levels were estimated in the brains of the kindled animals.
RESULTS
MSSE significantly increased the latency to onset of myoclonic jerks and the duration of clonic convulsions following PTZ administration. The MSSE pretreated group showed significantly reduced mean seizure score on PTZ-induced kindling. There was a significant increase in the brain MDA levels and decrease in GSH levels in response to PTZ-induced kindling. On MSSE pretreatment, there was a significant decrease in the MDA levels in the brains, though the increase in the GSH levels was not significant.
CONCLUSION
The results from this study suggest the presence of significant anticonvulsant activity in MSSE, in both acute and chronic PTZ-induced seizure models, which could be due to its antioxidant activity, as is reflected by the change in oxidative stress markers in brain.
SUMMARY
Evaluation of the anticonvulsant activity of and its effects on various markers of oxidative stress in the brain has not been done previously to the best of our knowledge stem extract (MSSE) significantly increased the latency to onset of myoclonic jerks and the duration of clonic convulsions in the experimental modelsThe MSSE pretreated group showed significantly reduced mean seizure score on pentylenetetrazole (PTZ)-induced kindlingThere was significant increase in the brain malondialdehyde (MDA) levels and decrease in glutathione (GSH) levels in response to PTZ-induced kindlingOn MSSE pretreatment, there was a significant decrease in the MDA levels in the brain, though the increase in the GSH levels was not significant. MSSE: stem extract, PTZ: Pentylenetetrazole, MES: Maximal electroshock seizures, MDA: Malondialdehyde, GSH: Glutathione, SOD: Superoxide dismutase, THLE: Tonic hindlimb extension.
背景
在先前的研究中，（香蕉）植物提取物已显示出具有抗氧化活性。氧化应激导致的神经元损伤是癫痫发病机制中的一个重要因素。
目的
本研究旨在评估茎提取物（MSSE）在小鼠急性和慢性实验模型中的抗惊厥活性，以及其对戊四氮（PTZ）点燃动物大脑中各种氧化应激标志物的影响。
材料与方法
急性研究采用最大电休克惊厥（MES）和PTZ诱导惊厥模型。对于慢性研究，研究了MSSE对点燃发展的影响。为评估MSSE对大脑氧化应激的影响，在点燃动物的大脑中估计丙二醛（MDA）和还原型谷胱甘肽（GSH）水平。
结果
MSSE显著增加了PTZ给药后肌阵挛性抽搐发作的潜伏期和阵挛性惊厥的持续时间。MSSE预处理组在PTZ诱导的点燃中平均癫痫发作评分显著降低。PTZ诱导点燃后，大脑MDA水平显著升高，GSH水平降低。MSSE预处理后，大脑中MDA水平显著降低，尽管GSH水平的升高不显著。
结论
本研究结果表明，在急性和慢性PTZ诱导的癫痫发作模型中，MSSE均具有显著的抗惊厥活性，这可能归因于其抗氧化活性，大脑中氧化应激标志物的变化反映了这一点。
总结
据我们所知，此前尚未对茎提取物（MSSE）的抗惊厥活性及其对大脑中各种氧化应激标志物的影响进行评估。在实验模型中，MSSE显著增加了肌阵挛性抽搐发作的潜伏期和阵挛性惊厥的持续时间。MSSE预处理组在戊四氮（PTZ）诱导的点燃中平均癫痫发作评分显著降低。PTZ诱导点燃后，大脑丙二醛（MDA）水平显著升高，谷胱甘肽（GSH）水平降低。MSSE预处理后，大脑中MDA水平显著降低，尽管GSH水平的升高不显著。MSSE：茎提取物，PTZ：戊四氮，MES：最大电休克惊厥，MDA：丙二醛，GSH：谷胱甘肽，SOD：超氧化物歧化酶，THLE：强直性后肢伸展
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