Barathi Selvaraj, Aruljothi K N, Karthik Chinnannan, Padikasan Indra Arulselvi
School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Plant and Microbial Biotechnology Laboratory, Department of Biotechnology, School of Biosciences, Periyar University, Salem, 636 011, Tamil Nadu, India.
Biotechnol Rep (Amst). 2020 Aug 31;28:e00522. doi: 10.1016/j.btre.2020.e00522. eCollection 2020 Dec.
The bacterial strain capable of decolorization and detoxification of the Reactive Blue 160 dye was isolated from a dye waste disposal site of Tirupur textile industries. The bacterial strain was screened and selected based on its decolorization capability of RB 160dye, which was identified as by 16S rRNA sequencing. The strain was tested for the decolorization potential under different physio-chemical experimental conditions (pH, temperature, agitation, non-agitation) and observed a complete decolorization at pH 7 and 35 °C under shaking condition within 48 h of time. The enzymes such as, Lignin peroxidase, azoreductase and NADH-DCI were significantly induced in the strain during the decolorization of RB160 dye. Phytotoxicity and microbial toxicity studies revealed that the decolorized product of RB160 dye is less toxic to the plants and microbes. Thus, our results recommend the prospective use of in bioremediation of RB160 dye.
能够对活性蓝160染料进行脱色和解毒的细菌菌株是从蒂鲁布尔纺织工业的染料废物处理场分离出来的。该细菌菌株基于其对RB 160染料的脱色能力进行筛选和选择，并通过16S rRNA测序进行鉴定。在不同的物理化学实验条件（pH值、温度、搅拌、不搅拌）下测试了该菌株的脱色潜力，发现在pH值为7、温度为35°C、振荡条件下，48小时内可实现完全脱色。在RB160染料脱色过程中，该菌株中木质素过氧化物酶、偶氮还原酶和NADH-DCI等酶被显著诱导。植物毒性和微生物毒性研究表明，RB160染料的脱色产物对植物和微生物的毒性较小。因此，我们的结果推荐了其在RB160染料生物修复中的潜在应用。
