Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
Biodegradation. 2010 Jun;21(3):453-63. doi: 10.1007/s10532-009-9315-6. Epub 2009 Nov 24.
A newly isolated novel bacterium from sediments contaminated with dyestuff was identified as Pseudomonas aeruginosa strain BCH by 16S rRNA gene sequence analysis. The bacterium was extraordinarily active and operative over a wide rage of temperature (10-60 degrees C) and salinity (5-6%), for decolorization of Direct Orange 39 (Orange TGLL) at optimum pH 7. This strain was capable of decolorizing Direct Orange 39; 50 mg l(-1) within 45 +/- 5 min, with 93.06% decolorization, while maximally it could decolorize 1.5 g l(-1) of dye within 48 h with 60% decolorization. Analytical studies as, UV-Vis spectroscopy, FTIR, HPLC were employed to confirm the biodegradation of dye and formation of new metabolites. Induction in the activities of lignin peroxidases, DCIP reductase as well as tyrosinase was observed, indicating the significant role of these enzymes in biodegradation of Direct Orange 39. Toxicity studies with Phaseolus mungo and Triticum aestivum revealed the non-toxic nature of degraded metabolites.
从受染料污染的沉积物中分离到的一种新型新型细菌，通过 16S rRNA 基因序列分析被鉴定为铜绿假单胞菌 BCH 株。该细菌在温度（10-60°C）和盐度（5-6%）的宽范围内都具有极高的活性和作用，可在最佳 pH 值 7 下对直接橙 39（橙色 TGLL）进行脱色。该菌株能够在 45 +/- 5 分钟内将 50 mg l(-1)的直接橙 39 脱色，脱色率为 93.06%，而在 48 小时内，其最大脱色能力为 1.5 g l(-1)，脱色率为 60%。采用 UV-Vis 光谱、FTIR、HPLC 等分析研究方法证实了染料的生物降解和新代谢物的形成。观察到木质素过氧化物酶、DCIP 还原酶和酪氨酸酶活性的诱导，表明这些酶在直接橙 39 的生物降解中起着重要作用。用绿豆和小麦进行的毒性研究表明，降解代谢物无毒性。
