Sensitive determination of NT-proBNP for diagnosing abdominal aortic aneurysms incidence on interdigitated electrode sensor.

Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immediate treatment. Blood-based biomarker helps to diagnose AAA and to monitor the condition after AAA surgery. N-terminal pro-B-type natriuretic peptide (NT-proBNP) is a hormone produced in the heart in small quantities and increased when the heart needs to work harder. NT-proBNP was proved to be strongly linked with AAA incidence. Moreover, quantifying the level of NT-proBNP helps to determine the risk factors on cardiovascular system after the surgery. This work is quantifying the NT-proBNP on interdigitated electrode sensor by using NT-proBNP binding aptamer. The detection limit of NT-proBNP was calculated as 1 pg/mL on a linear regression curve [y = 0.2148x + 0.8849; R² = 0.9049]. The linear range with dose-dependent analysis was from 0.01 until 100 ng/mL. Moreover, the control experiment with complementary aptamer sequence did not show the current signal, specifying the detection of NT-proBNP. This research benefits to identify the heart condition of patient after the removal of AAA.