Qiu Changcheng, Wang Xiao, Zhang Xia, Li Zhenhuan, Zhou Yiming, Kang Jie
Department of General Surgery, People's Hospital of Gaomi, Gaomi, Shandong Province, People's Republic of China.
Department of Vascular Surgery, Liaocheng People's Hospital, Liaocheng, Shandong Province, People's Republic of China.
Biotechnol Appl Biochem. 2021 Aug;68(4):865-870. doi: 10.1002/bab.2006. Epub 2020 Aug 25.
Abdominal aortic aneurysm (AAA) is a vascular disease found to have progressive growth in the area of aorta. Rupturing of aorta causes excessive bleeding that leads to health-related issues, which can be fatal sometimes. Therefore, it becomes important to make early diagnosis of AAA and its condition and start immediate treatment. Blood-based biomarker helps to diagnose AAA and to monitor the condition after AAA surgery. N-terminal pro-B-type natriuretic peptide (NT-proBNP) is a hormone produced in the heart in small quantities and increased when the heart needs to work harder. NT-proBNP was proved to be strongly linked with AAA incidence. Moreover, quantifying the level of NT-proBNP helps to determine the risk factors on cardiovascular system after the surgery. This work is quantifying the NT-proBNP on interdigitated electrode sensor by using NT-proBNP binding aptamer. The detection limit of NT-proBNP was calculated as 1 pg/mL on a linear regression curve [y = 0.2148x + 0.8849; R² = 0.9049]. The linear range with dose-dependent analysis was from 0.01 until 100 ng/mL. Moreover, the control experiment with complementary aptamer sequence did not show the current signal, specifying the detection of NT-proBNP. This research benefits to identify the heart condition of patient after the removal of AAA.
腹主动脉瘤（AAA）是一种在主动脉区域发现有进行性生长的血管疾病。主动脉破裂会导致大量出血，进而引发与健康相关的问题，有时甚至会危及生命。因此，早期诊断AAA及其病情并立即开始治疗变得至关重要。基于血液的生物标志物有助于诊断AAA并监测AAA手术后的病情。N端前B型利钠肽（NT-proBNP）是一种在心脏中少量产生的激素，当心脏需要更努力工作时其水平会升高。事实证明，NT-proBNP与AAA发病率密切相关。此外，量化NT-proBNP水平有助于确定手术后心血管系统的风险因素。这项工作通过使用NT-proBNP结合适体在叉指电极传感器上对NT-proBNP进行定量。在一条线性回归曲线[y = 0.2148x + 0.8849；R² = 0.9049]上计算出NT-proBNP的检测限为1 pg/mL。剂量依赖性分析的线性范围为0.01至100 ng/mL。此外，用互补适体序列进行的对照实验未显示电流信号，这表明对NT-proBNP的检测具有特异性。这项研究有助于在切除AAA后识别患者的心脏状况。
