Labiom-R (Analytical Laboratory of Restorative Biomaterials), Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Department of Restorative Dentistry, Division of Biomaterials and Biomechanics, Oregon Health and Science University, Portland, Oregon, USA.
J Biomed Mater Res B Appl Biomater. 2021 Mar;109(3):428-435. doi: 10.1002/jbm.b.34711. Epub 2020 Aug 25.
This study evaluated the biological behavior of the coffee compounds Trigonelline (T), chlorogenic acid (C), and nicotinic acid (N), correlating with their release from a resin matrix. Minimum inhibitory concentration (MIC) was evaluated against Streptococcus mutans UA159, and cytotoxicity was assessed by methyl tetrazolium salt on OD-21 cells. Resin matrices (bisphenol A-glycidyl-dimethacrylate/triethylene glycol-dimethacrylate 70/30 wt%, camphorquinone/ethyl 4-dimethyl aminobenzoate 0.5/1 wt%) were doped with coffee compounds in different concentrations (10/20/30/40/50 wt%), performing 15 groups (T10-T50, C10-C50, N10-N50), and a control group with no coffee compound. Degree of conversion (DC%) was analyzed by Fourier transform infrared spectroscopy. Antimicrobial properties were evaluated by bioluminescence (Luciferase assay). The release from loaded matrices was analyzed over time (24 hr, 6, 14, 21 and 28 days), using high-performance liquid chromatography (HPLC). Data were submitted to ANOVA/Tukey's test (α = 0.05). MIC for T and C was 6 mg/ml, and 4 mg/ml for N. None of them were cytotoxic. Only T50 and C50 showed lower DC% than control (α < 0.05). Some groups (T30/T40/T50/C40/C50/N50) were strongly antimicrobial, reducing bacterial activity approximately five times compared to control (α < 0.05). For T30, T40, T50, C40, and C50, the HPLC showed a release above or closer to MIC values mainly in 24 hr, but for N50, up to 28 days. In conclusion, the coffee compounds presented antimicrobial activity, depending on their concentration when added in resin matrices, being found a correlation with their release.
本研究评估了咖啡化合物中的瓜氨酸（T）、绿原酸（C）和烟酸（N）的生物学行为，并将其与从树脂基质中释放出来的情况进行了关联。采用最小抑菌浓度（MIC）法评估了它们对变异链球菌 UA159 的抑制作用，并用甲基噻唑基四唑盐（MTT）法在 OD-21 细胞上评估了它们的细胞毒性。树脂基质（双酚 A-缩水甘油二甲基丙烯酸酯/三乙二醇二甲基丙烯酸酯 70/30wt%，樟脑醌/乙基 4-二甲氨基苯甲酸酯 0.5/1wt%）中以不同浓度（10/20/30/40/50wt%）掺杂了咖啡化合物，共进行了 15 组实验（T10-T50、C10-C50、N10-N50），以及一组不含咖啡化合物的对照组。采用傅里叶变换红外光谱法（FTIR）分析了转化率（DC%）。通过生物发光（荧光素酶测定法）评估了抗菌性能。通过高效液相色谱法（HPLC）在不同时间（24 小时、6、14、21 和 28 天）分析了负载基质的释放情况。数据采用方差分析/Tukey 检验（α=0.05）进行处理。T 和 C 的 MIC 为 6mg/ml，N 的 MIC 为 4mg/ml。它们均无细胞毒性。只有 T50 和 C50 的 DC%低于对照组（α<0.05）。一些组（T30/T40/T50/C40/C50/N50）具有很强的抗菌活性，与对照组相比，细菌活性降低了约五倍（α<0.05）。对于 T30、T40、T50、C40 和 C50，HPLC 显示主要在 24 小时内释放出高于或接近 MIC 值的物质，但对于 N50，则持续到 28 天。综上所述，咖啡化合物具有抗菌活性，且与它们在树脂基质中的浓度有关，同时也与它们的释放情况相关。
