Department of Urology Surgery, Caoxian People's Hospital, Heze,
Eur Rev Med Pharmacol Sci. 2020 Sep;24(17):8740-8746. doi: 10.26355/eurrev_202009_22812.
This study aims to uncover the in vitro influences of lncRNA TMPO-AS1 on the progression of bladder cancer (BLCA) and the underlying mechanism.
Expression levels of TMPO-AS1 in BLCA tissues and normal bladder tissues were analyzed in The Cancer Genome Atlas (TCGA) database. Differential expressions of TMPO-AS1 in BLCA tissues and normal bladder epithelial tissues were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Potential influence of TMPO-AS1 on prognosis of BLCA patients was assessed. In vitro influences of TMPO-AS1 on proliferative and migratory abilities in T24 and UMUC-3 cells were evaluated by Cell Counting Kit-8 (CCK-8), transwell, and wound healing assay, respectively. Finally, the correlation between TMPO-AS1 and its sense RNA TMPO was assessed by analyzing TCGA database, clinical samples, and BLCA cell lines.
By analyzing TCGA database and clinical samples, it was found that TMPO-AS1 was upregulated in BLCA tissues compared with that in normal bladder tissues. Worse survival was observed in BLCA patients with high expression of TMPO-AS1. TMPO-AS1 level was correlated to muscle invasiveness and TNM stage of BLCA patients. In T24 and UMUC-3 cells, the knockdown of TMPO-AS1 suppressed proliferative and migratory abilities. TMPO-AS1 level was positively correlated to that of its sense RNA TMPO. Moreover, the knockdown of TMPO-AS1 downregulated mRNA and protein levels of TMPO in BLCA cells.
TMPO-AS1 is upregulated in BLCA tissue and closely linked to poor prognosis of BLCA patients.
本研究旨在揭示 lncRNA TMPO-AS1 对膀胱癌（BLCA）进展的体外影响及其潜在机制。
在癌症基因组图谱（TCGA）数据库中分析 BLCA 组织和正常膀胱组织中 TMPO-AS1 的表达水平。通过定量实时聚合酶链反应（qRT-PCR）检测 BLCA 组织和正常膀胱上皮组织中 TMPO-AS1 的差异表达。评估 TMPO-AS1 对 BLCA 患者预后的潜在影响。通过细胞计数试剂盒-8（CCK-8）、Transwell 和划痕愈合实验分别评估 TMPO-AS1 对 T24 和 UMUC-3 细胞增殖和迁移能力的体外影响。最后，通过分析 TCGA 数据库、临床样本和 BLCA 细胞系评估 TMPO-AS1 与其 sense RNA TMPO 之间的相关性。
通过分析 TCGA 数据库和临床样本，发现与正常膀胱组织相比，BLCA 组织中 TMPO-AS1 表达上调。TMPO-AS1 高表达的 BLCA 患者生存情况较差。TMPO-AS1 水平与 BLCA 患者的肌肉浸润和 TNM 分期相关。在 T24 和 UMUC-3 细胞中，TMPO-AS1 的敲低抑制了增殖和迁移能力。TMPO-AS1 水平与 TMPO 的 sense RNA 呈正相关。此外，TMPO-AS1 的敲低下调了 BLCA 细胞中 TMPO 的 mRNA 和蛋白水平。
TMPO-AS1 在 BLCA 组织中上调，与 BLCA 患者的不良预后密切相关。
