Cátedra "B" de Química Biológica, Facultad de Odontología, Universidad Nacional de Córdoba, Córdoba, Argentina.
INICSA/UNC-CONICET, Enrique Barros esquina Enfermera Gordillo, Ciudad Universitaria, Córdoba, Argentina.
J Periodontal Res. 2021 Jan;56(1):154-161. doi: 10.1111/jre.12806. Epub 2020 Sep 23.
Cellular damage related to oxidative stress (OS) is implicated in periodontal diseases (PD). Melatonin (MEL) has multiple functions, and it has been described as a potential treatment for PD. We aim at evaluating the protective effects of MEL on an in vitro model of cellular damage triggered by glutamate (GLUT) and DL-buthionine sulfoximine (BSO), on gingival cells (GCs) in culture.
A primary culture of GCs from Wistar rats was developed in order to test the protective property of MEL; BSO and GLUT were administered alone as well as in combination with MEL. The viability and apoptosis were measured with MTT assay and TUNEL, respectively, and the concentration of superoxide anion ( ) was measured with the NBT method.
The combination of BSO and GLUT treatment resulted in a decreased viability of GCs. This was evidenced by the increase in both the production of superoxide anion and apoptosis. After MEL administration, the oxidant and pro-apoptotic effects of BSO and GLUT were totally counteracted.
These findings demonstrated that MEL has an effective protective role on GCs subjected to cellular damage in a model of OS and cytotoxicity triggered by BSO and GLUT. Consequently, MEL could be used as a therapeutic agent in PD which begin with a significative loss of GCs.
氧化应激（OS）引起的细胞损伤与牙周病（PD）有关。褪黑素（MEL）具有多种功能，被描述为 PD 的潜在治疗方法。我们旨在评估 MEL 对谷氨酸（GLUT）和 DL-丁硫氨酸亚砜（BSO）诱导的体外细胞损伤模型中培养的牙龈细胞（GCs）的保护作用。
为了测试 MEL 的保护特性，我们开发了来自 Wistar 大鼠的 GCs 原代培养物；单独给予 BSO 和 GLUT 以及与 MEL 联合给予 BSO 和 GLUT。使用 MTT 测定法测量细胞活力和细胞凋亡，使用 NBT 法测量超氧阴离子（ ）的浓度。
BSO 和 GLUT 联合处理导致 GCs 活力下降。这表现在超氧阴离子的产生和细胞凋亡的增加。给予 MEL 后，BSO 和 GLUT 的氧化和促凋亡作用完全被抵消。
这些发现表明，MEL 在 BSO 和 GLUT 诱导的 OS 和细胞毒性模型中对受到细胞损伤的 GCs 具有有效保护作用。因此，MEL 可用于以 GCs 大量丧失为特征的 PD 治疗。
