Areco Vanessa, Rodriguez Valeria, Marchionatti Ana, Carpentieri Agata, Tolosa de Talamoni Nori
Laboratorio "Dr. Fernando Cañas", Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, INICSA (CONICET-Universidad Nacional de Córdoba), Pabellón Argentina, 2do. Piso, Ciudad Universitaria, 5000 Córdoba, Argentina.
Laboratorio "Dr. Fernando Cañas", Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, INICSA (CONICET-Universidad Nacional de Córdoba), Pabellón Argentina, 2do. Piso, Ciudad Universitaria, 5000 Córdoba, Argentina; Química Biológica, Facultad de Odontología, Universidad Nacional de Córdoba, Córdoba, Argentina.
Comp Biochem Physiol A Mol Integr Physiol. 2016 Jul;197:16-22. doi: 10.1016/j.cbpa.2016.03.005. Epub 2016 Mar 9.
We have previously demonstrated that melatonin (MEL) blocks the inhibition of the intestinal Ca(2+) absorption caused by menadione (MEN). The purpose of this study were to determine whether MEL not only restores but also prevents the intestinal Ca(2+) absorption inhibited either by MEN or BSO, two drugs that deplete glutathione (GSH) in different ways, and to analyze the mechanisms by which MEN and MEL alter the movement of Ca(2+) across the duodenum. To know this, chicks were divided into four groups: 1) controls, 2) MEN treated, 3) MEL treated, and 4) treated sequentially with MEN and MEL or with MEN and MEL at the same time. In a set of experiments, chicks treated with BSO or sequentially with BSO and MEL or with BSO and MEL at the same time were used. MEL not only restored but also prevented the inhibition of the chick intestinal Ca(2+) absorption produced by either MEN or BSO. MEN altered the protein expression of molecules involved in the transcellular as well as in the paracellular pathway of the intestinal Ca(2+) absorption. MEL restored partially both pathways through normalization of the O2(-) levels. The nitrergic system was not altered by any treatment. In conclusion, MEL prevents or restores the inhibition of the intestinal Ca(2+) absorption caused by different GSH depleting drugs. It might become one drug for the treatment of intestinal Ca(2+) absorption under oxidant conditions having the advantage of low or null side effects.
我们之前已经证明褪黑素（MEL）可阻断甲萘醌（MEN）对肠道钙（Ca²⁺）吸收的抑制作用。本研究的目的是确定MEL是否不仅能恢复，还能预防由MEN或丁硫氨酸亚砜胺（BSO）抑制的肠道Ca²⁺吸收，这两种药物以不同方式消耗谷胱甘肽（GSH），并分析MEN和MEL改变Ca²⁺跨十二指肠转运的机制。为了了解这一点，将雏鸡分为四组：1）对照组，2）MEN处理组，3）MEL处理组，4）先MEN后MEL处理组或MEN与MEL同时处理组。在一组实验中，使用了用BSO处理的雏鸡，或先BSO后MEL处理组或BSO与MEL同时处理组。MEL不仅恢复了，而且预防了由MEN或BSO对雏鸡肠道Ca²⁺吸收的抑制。MEN改变了参与肠道Ca²⁺吸收的跨细胞和细胞旁途径的分子的蛋白质表达。MEL通过使超氧阴离子（O₂⁻）水平正常化部分恢复了这两条途径。任何处理均未改变一氧化氮能系统。总之，MEL预防或恢复了由不同GSH消耗药物引起的肠道Ca²⁺吸收抑制。它可能成为一种治疗氧化应激条件下肠道Ca²⁺吸收的药物，具有低副作用或无副作用的优势。
