Department of Plant Production, Graduate Program in Agricultural and Livestock Microbiology, São Paulo State University (UNESP), School of Agricultural and Veterinarian Sciences, Access Way Prof. Paulo Donato Castellane, Jaboticabal, 14884-900, Brazil.
A & L Biologicals, Agroecological Research Service Center, London, ON, Canada.
Curr Microbiol. 2020 Dec;77(12):3878-3887. doi: 10.1007/s00284-020-02207-9. Epub 2020 Sep 23.
The use of chemical fertilizers strongly promotes productivity in agricultural crops; therefore, large amounts of chemical fertilizers have been used. The use of plant growth-promoting bacteria may be a strategy to reduce the use of chemical fertilizers; however, little is known about the effect of chemical fertilization on the performance of these bacteria through plant-microbe interactions. The present study aimed to verify the performance of Bacillus subtilis, Azospirillum brasilense, B. pumilus, B. amyloliquefaciens, Herbaspirillum seropedicae, Gluconacetobacter diazotrophicus, and the mixtures A. brasilense + B. subtilis, B. pumilus + B. amyloliquefaciens, and H. seropedicae + G. diazotrophicus on parameters such as nitrogen and phosphorus extraction from soil, the concentrations of these nutrients in maize plants, and plant growth in both fertilized and unfertilized soil. The results showed that H. seropedica increased the nitrogen content by 6.6 g kg in leaves and 2.2 g kg in the root when comparing the unfertilized with the fertilized condition. G. diazotrophicus increased the nitrogen content by 3.7 g kg in leaves and 2.4 g kg in the root. B. pumilus increased the phosphorous content by 1.7 g kg in leaves, and B. amyloliquefaciens increased the phosphorous content by 0.61 g kg. The present study showed that even though the bacteria presented good performance related to plant growth under fertilized conditions, H. seropedicae, G. diazotrophicus, B. pumilus, and B. amyloliquefaciens could be used in the maize crop with a reduced chemical fertilization dose.
化肥的使用强烈促进了农作物的生产力；因此，大量的化肥被使用。使用植物生长促进细菌可能是减少化肥使用的一种策略；然而，关于化肥对这些细菌通过植物-微生物相互作用的性能的影响知之甚少。本研究旨在验证枯草芽孢杆菌、巴西固氮螺菌、解淀粉芽孢杆菌、解淀粉类芽孢杆菌、草螺菌、谷氨酸棒杆菌和巴西固氮螺菌+枯草芽孢杆菌、解淀粉芽孢杆菌+解淀粉类芽孢杆菌、草螺菌+谷氨酸棒杆菌混合物在从土壤中提取氮和磷、这些养分在玉米植株中的浓度以及施肥和未施肥土壤中的植物生长等方面的性能。结果表明，与未施肥相比，草螺菌使叶片中的氮含量增加了 6.6g/kg，根中的氮含量增加了 2.2g/kg。谷氨酸棒杆菌使叶片中的氮含量增加了 3.7g/kg，根中的氮含量增加了 2.4g/kg。解淀粉芽孢杆菌使叶片中的磷含量增加了 1.7g/kg，解淀粉类芽孢杆菌使叶片中的磷含量增加了 0.61g/kg。本研究表明，即使细菌在施肥条件下表现出良好的生长性能，但草螺菌、谷氨酸棒杆菌、解淀粉芽孢杆菌和解淀粉类芽孢杆菌仍可用于玉米作物，减少化学施肥量。
