Sato K, Sato F
Am J Physiol. 1987 Jun;252(6 Pt 2):R1099-105. doi: 10.1152/ajpregu.1987.252.6.R1099.
Na+, K+, Cl-, and HCO-3 concentrations were determined in both methacholine (MCh)-induced and isoproterenol (ISO)-induced primary sweat collected directly from isolated and cannulated rhesus monkey palm eccrine secretory coils in vitro. Na+ concentration [( Na+]) of MCh-induced sweat was higher than that of the bathing medium by 4.2 mM, i.e., 155.4 vs. 151.2 mM. Sweat [Cl-] was consistently higher than that of the medium by 13.7 and 11.2 mM in both MCh- and ISO-induced primary fluid, respectively. The sweat-to-bath Cl- gradient increased as [Cl-] in the bath was lowered by substituting with less-permeable anions. In contrast sweat [HCO-3] was much lower in both MCh- and ISO-induced sweat than that of the bathing medium, i.e., approximately 6 mM in sweat vs. 25 mM in the bath. [K+] in MCh-induced primary sweat (mean of 6.63 mM) was consistently higher than that of the bathing medium (5 mM). Furthermore, [K+] tended to be the highest in the first sweat sample after MCh stimulation, reaching as high as 9 mM. In sharp contrast ISO-induced primary sweat showed [K+] that was almost always less than 5 mM with the mean of 4.03 mM. These electrolyte profiles of the primary fluid have been discussed relative to the transport model generally applied for Cl- secretory epithelia.
在体外，从分离并插管的恒河猴手掌小汗腺分泌盘直接收集的乙酰甲胆碱（MCh）诱导的和异丙肾上腺素（ISO）诱导的原发性汗液中，测定了Na⁺、K⁺、Cl⁻和HCO₃⁻的浓度。MCh诱导的汗液中Na⁺浓度[（Na⁺）]比浴液高4.2 mM，即155.4 mM对151.2 mM。在MCh诱导的和ISO诱导的原发性汗液中，汗液[Cl⁻]分别始终比浴液高13.7 mM和11.2 mM。随着用渗透性较低的阴离子替代浴液中的[Cl⁻]，汗液与浴液之间的Cl⁻梯度增加。相比之下，MCh诱导的和ISO诱导的汗液中[HCO₃⁻]均远低于浴液，即汗液中约为6 mM，而浴液中为25 mM。MCh诱导的原发性汗液中的[K⁺]（平均为6.63 mM）始终高于浴液（5 mM）。此外，MCh刺激后的第一个汗液样本中[K⁺]往往最高，高达9 mM。与之形成鲜明对比的是，ISO诱导的原发性汗液中的[K⁺]几乎总是低于5 mM，平均为4.03 mM。这些原发性汗液的电解质特征已相对于通常应用于Cl⁻分泌上皮的转运模型进行了讨论。
