Sato K, Sato F
Am J Physiol. 1981 Sep;241(3):C113-20. doi: 10.1152/ajpcell.1981.241.3.C113.
The role of Ca2+ in eccrine sweat secretion was studied using isolated cannulated monkey palm eccrine sweat glands in vitro. Removal of Ca2+ from the incubation medium promptly abolished sweat secretion induced by methacholine or phenylephrine. In contrast, isoproterenol-induced sweat secretion lasted from 40 to 220 min in a Ca2+-free medium. The methacholine-induced maximal sweat rate was a hyperbolic function of the Ca2+ concentration in the bath and reached a plateau at 1 mM Ca2+. Higher Ca2+ concentrations rather suppressed the secretory rate. The Ca2+ ionophore A23187, but not X537A, at 3 X 10(-6) M induced copious prolonged sweat secretion after a latent period of 10 min. A23187-induced sweat secretion was not inhibited by either atropine or propranolol. D 600 (methoxyverapamil) at 10(-3) M inhibited sweat secretion induced by methacholine or by isoproterenol, although the latter lasted longer than methacholine sweating (20 vs. 5 min) in the presence of D 600. The data support the notion that Ca2+ influx into the cell plays a crucial role in cholinergic and alpha-adrenergic sweating, whereas a partial supply of Ca2+ for isoproterenol-induced sweating is derived from an intracellular store.
利用体外分离的插管猴掌小汗腺研究了Ca2+在小汗腺汗液分泌中的作用。从孵育培养基中去除Ca2+可迅速消除由乙酰甲胆碱或去氧肾上腺素诱导的汗液分泌。相比之下，在无Ca2+培养基中，异丙肾上腺素诱导的汗液分泌持续40至220分钟。乙酰甲胆碱诱导的最大汗液分泌率是浴液中Ca2+浓度的双曲线函数，在1 mM Ca2+时达到平台期。较高的Ca2+浓度反而会抑制分泌率。3×10(-6) M的Ca2+离子载体A23187（而非X537A）在10分钟的潜伏期后可诱导大量持续的汗液分泌。A23187诱导的汗液分泌不受阿托品或普萘洛尔的抑制。10(-3) M的D 600（甲氧基维拉帕米）可抑制乙酰甲胆碱或异丙肾上腺素诱导的汗液分泌，尽管在D 600存在的情况下，异丙肾上腺素诱导的汗液分泌持续时间比乙酰甲胆碱诱导的汗液分泌更长（分别为20分钟和5分钟）。这些数据支持以下观点：Ca2+流入细胞在胆碱能和α-肾上腺素能性出汗中起关键作用，而异丙肾上腺素诱导出汗的部分Ca2+供应来自细胞内储存库。
