Knych E T
Alcohol Clin Exp Res. 1987 Apr;11(2):112-7. doi: 10.1111/j.1530-0277.1987.tb01273.x.
After 2 days of intoxication an endothelium-dependent tolerance to ethanol-induced contraction of the rat aorta develops. This study investigates the possibility that the observed tolerance may be due to the recently described endothelium-dependent vasodilator system which mediates the action of several potent vasodilators. The maximum response induced by ethanol in control and intoxicated aortic rings with endothelium was significantly increased by treatment with eicosatetraynoic acid and methylene blue. In addition, these agents significantly decreased the EC50 of ethanol in intoxicated but not control rings with endothelium. The response of control and intoxicated aortic rings without endothelium was unaltered by eicosatetraynoic acid or methylene blue. Indomethacin, nordihydroguaiaretic acid, and FPL 55712 did not alter either the maximum response or the EC50 of ethanol in control or intoxicated rings with endothelium. These observations support the hypothesis that the endothelium-dependent tolerance observed in the aorta may be due to the release of an endothelium-derived relaxing factor.
在乙醇中毒2天后，大鼠主动脉对乙醇诱导的收缩产生内皮依赖性耐受。本研究探讨了观察到的耐受性可能是由于最近描述的介导几种强效血管舒张剂作用的内皮依赖性血管舒张系统。用二十碳四炔酸和亚甲蓝处理后，乙醇在有内皮的对照和中毒主动脉环中诱导的最大反应显著增加。此外，这些药物显著降低了乙醇在有内皮的中毒环而非对照环中的半数有效浓度（EC50）。二十碳四炔酸或亚甲蓝对无内皮的对照和中毒主动脉环的反应无影响。吲哚美辛、去甲二氢愈创木酸和FPL 55712对有内皮的对照或中毒环中乙醇的最大反应或EC50均无改变。这些观察结果支持这样的假设，即主动脉中观察到的内皮依赖性耐受可能是由于内皮源性舒张因子的释放。
