Knych E T, Guimaraes C M, Boivin S
Life Sci. 1984 Aug 6;35(6):611-7. doi: 10.1016/0024-3205(84)90256-x.
Ethanol, at high concentrations, produced a dose-dependent contraction of male rat aortic rings, in vitro. Mechanical removal of endothelial cells from aortic rings of control rats resulted in a small, but significant, shift of the ethanol dose-response curve to the right without a change in the maximal contraction. Removing the endothelial cells of aortic rings obtained from rats intoxicated with ethanol for two days significantly shifted the ethanol dose-response curve to the left and significantly increased the maximal contraction induced by ethanol. A comparison of the ethanol dose-response curves in aortic rings with endothelium obtained from control rats with those obtained from intoxicated rats indicated a significant shift to the right with no change in maximal response. No significant changes were observed when the responses of aortic rings without endothelium obtained from control and intoxicated rats were compared. These observations confirm that tolerance to ethanol can be demonstrated in vascular smooth muscle. In addition, they demonstrate that the endothelium is required for the development of tolerance to ethanol in the aorta.
在体外实验中，高浓度乙醇可使雄性大鼠主动脉环产生剂量依赖性收缩。从对照大鼠的主动脉环机械去除内皮细胞后，乙醇剂量-反应曲线轻微但显著地右移，最大收缩幅度不变。去除经两天乙醇中毒的大鼠的主动脉环内皮细胞后，乙醇剂量-反应曲线显著左移，且乙醇诱导的最大收缩幅度显著增加。将对照大鼠具有内皮的主动脉环的乙醇剂量-反应曲线与中毒大鼠的进行比较，结果显示曲线显著右移，最大反应无变化。比较对照和中毒大鼠无内皮的主动脉环的反应，未观察到显著变化。这些观察结果证实，血管平滑肌中可表现出对乙醇的耐受性。此外，它们还表明，主动脉中对乙醇耐受性的形成需要内皮细胞。
