空肠袢位置对4至5周龄仔猪大肠杆菌热稳定肠毒素活性的影响。

Effect of jejunal loop location on the activity of Escherichia coli heat-stable enterotoxin in 4- to 5-week-old pigs.

作者信息

Dove C R, Zinn G M, Veum T L, Ellersieck M R, Parker C D, White A A

出版信息

Am J Vet Res. 1987 Apr;48(4):558-61.

Abstract

Heat-stable enterotoxin (STa) from Escherichia coli strain 431 was injected into the jejunum of 4 pigs from each of 3 litters, using a ligated intestinal loop assay (with loops beginning 1 m caudad to the pylorus). The jejunum was divided into 4 contiguous areas, with 4 loops in each area. Doses of 0, 10, 100, or 1,000 ng of purified toxin (10 ng/mouse unit) were injected into the loops within an area, using a 4 X 4 Latin square design. Fluid accumulation in the loops increased (P less than 0.05) with increasing concentrations of STa in pigs in all litters, but the magnitude of the response varied across litters. Fluid responses to the STa varied in the different areas of the jejunum, with pigs in 2 litters having a decrease (P less than 0.05) in the response to STa in the caudal areas. These data quantitate the variability within the different areas of the jejunum of the young pig.

摘要

利用结扎肠袢试验（肠袢起始于幽门尾侧1米处），将来自大肠杆菌431菌株的热稳定肠毒素（STa）注入3窝仔猪中每窝的4头仔猪的空肠。空肠被分成4个相邻区域，每个区域有4个肠袢。采用4×4拉丁方设计，将0、10、100或1000纳克纯化毒素（10纳克/小鼠单位）注入一个区域内的肠袢中。在所有窝的仔猪中，随着STa浓度的增加，肠袢中的液体蓄积量增加（P<0.05），但不同窝的反应程度有所不同。空肠不同区域对STa的液体反应各不相同，2窝仔猪对尾侧区域STa的反应有所降低（P<0.05）。这些数据定量了幼猪空肠不同区域内的变异性。