Lucas M L, Duncan N W, o'reilly N F, McIlvenny T J, Nelson Y B
Laboratory of Gastrointestinal Physiology, Division of Neuroscience & Biomedical Systems, Institute of Biomedical & Life Science, Glasgow University, Glasgow, UK.
Neurogastroenterol Motil. 2008 May;20(5):532-8. doi: 10.1111/j.1365-2982.2007.01046.x. Epub 2008 Jan 7.
On contact with the mucosa, heat stable (STa) enterotoxin from Escherichia coli reduces fluid absorption in vivo in the perfused jejunum of the anaesthetized rat. The question of whether it also has a vagally mediated remote action on jejunal absorption, when instilled into the ileum, was re-examined, given contradictory findings in the literature. A standard perfused loop preparation was used to measure luminal uptake of fluid in vivo by means of volume recovery. STa in the ileum was found to have no effect on jejunal absorption, regardless of cervical or sub-diaphragmatic vagotomy and also regardless of the nature of the perfusate anion. The batches of toxin were shown in parallel experiments to reduce fluid absorption directly in the jejunum and also in the ileum. Similarly, vagal nerves prior to section had demonstrable in vivo physiological function. There was therefore no evidence for an indirect, vagally mediated ileal effect of STa on proximal fluid absorption.
与黏膜接触时，来自大肠杆菌的热稳定（STa）肠毒素会降低麻醉大鼠灌流空肠在体内的液体吸收。鉴于文献中的矛盾发现，当将其注入回肠时，它是否也对空肠吸收有迷走神经介导的远程作用这一问题被重新研究。使用标准的灌流肠袢制备方法，通过体积恢复来测量体内肠腔对液体的摄取。发现回肠中的STa对空肠吸收没有影响，无论是否进行颈迷走神经切断术或膈下迷走神经切断术，也无论灌流液阴离子的性质如何。在平行实验中表明，这些批次的毒素可直接降低空肠和回肠中的液体吸收。同样，切断迷走神经之前，迷走神经在体内具有可证明的生理功能。因此，没有证据表明STa对近端液体吸收有间接的、迷走神经介导的回肠作用。
