Pumarola-Sune T, Navia B A, Cordon-Cardo C, Cho E S, Price R W
Ann Neurol. 1987 May;21(5):490-6. doi: 10.1002/ana.410210513.
Human immunodeficiency virus infection was identified immunohistochemically in the brains of 8 patients with acquired immune deficiency syndrome dementia complex. Using a monoclonal antibody against a structural viral protein (p25), infection was detected in white matter and basal ganglia in a distribution paralleling that of the major neuropathological abnormalities. Viral antigen was identified principally in perivascular and parenchymal macrophages and in multinucleated cells of macrophage origin that were identified morphologically and by immunocytochemical staining for acid phosphatase isozyme. In 4 of the 8 patients, viral antigen was also detected in acid-phosphatase-negative, process-bearing neuroglial cells; in 2 patients, antigen was detected in basal ganglion cells that were morphologically consistent with neurons and in alkaline-phosphatase-positive cells with elongated nuclei that were most likely of endothelial origin.
通过免疫组织化学方法在8例获得性免疫缺陷综合征痴呆综合征患者的大脑中鉴定出人类免疫缺陷病毒感染。使用针对病毒结构蛋白（p25）的单克隆抗体，在白质和基底神经节中检测到感染，其分布与主要神经病理学异常的分布平行。病毒抗原主要在血管周围和实质巨噬细胞以及巨噬细胞来源的多核细胞中鉴定出来，这些细胞通过形态学和酸性磷酸酶同工酶的免疫细胞化学染色进行鉴定。在8例患者中的4例中，在酸性磷酸酶阴性、有突起的神经胶质细胞中也检测到病毒抗原；在2例患者中，在形态上与神经元一致的基底神经节细胞以及核呈细长形、碱性磷酸酶阳性且最可能为内皮来源的细胞中检测到抗原。
